摘要
为了分析谷子叶片表现黄化、卷曲症状的形成特点及原因,对谷子白发病菌侵染的谷子植株表型进行测定,并利用3,5-二硝基水杨酸(DNS)法测定了叶片4种细胞壁降解酶的活性。结果表明,受侵染植株的株高、叶面积、叶片厚度、叶绿素SPAD值、叶长、叶宽和叶表皮毛数量等表型指标与健康植株相比均有显著性差异;同时病叶中果胶甲酯酶(PME)和果胶裂解酶(PL)活性均显著高于健康叶,酶活性最高可分别达到585.23、275.55 U/g,而纤维素酶(CE)和多聚半乳糖醛酸酶(PG)活性与健康叶无显著性差异,酶活性最高可分别达到1467.04、931.34 U/g。谷子叶片受到侵染后会发生黄化、畸形,细胞壁降解酶在病原菌扩展及致病过程中发挥重要作用。
To analyze the formation characteristics and causes of the symptoms yellowing and curling in millet leaves,the phenotype of the millet plants infected by Sclerospora graminicola was determined,and the activities of 4 cell wall degrading enzymes were determined by the 3,5-dinitrosalicylic acid(DNS)method.The results showed that the phenotypic indicators such as plant height,leaf area,leaf thickness,chlorophyll SPAD value,leaf length,leaf width and the number of leaf epidermal hair of the infected plants were significantly different from those of healthy plants.At the same time,the activities of pectin methylesterase(PME)and pectin lyase(PL)in diseased leaves were significantly higher than healthy leaves,and the highest enzyme activities respectively reached 585.23,275.55 U/g.However,the activities of cellulase(CE)and polygalacturonase(PG)were no significant difference with healthy leaves,and the highest enzyme activity respectively reached 1467.04,931.34 U/g.Millet leaves are yellow and deformed after being infected,cell wall degrading enzyme will play an important role in the process of pathogen expansion and pathogenesis.
作者
刘琴
张蕊
张育铭
任璐
张宝俊
LIU Qin;ZHANG Rui;ZHANG Yuming;REN Lu;ZHANG Baojun(College of Plant Protection,Shanxi Agricultural University,Taigu 030801,China)
出处
《山西农业科学》
2021年第3期346-350,共5页
Journal of Shanxi Agricultural Sciences
基金
山西省高等学校大学生创新创业训练项目(2020123)。
关键词
谷子白发病
DNS法
症状观测
细胞壁降解酶
活性测定
foxtail millet downy mildew
DNS method
symptom observation
cell wall degrading enzyme
activity determination
