摘要
超分辨显微技术突破衍射极限的分辨能力为研究纳米尺度的超精细结构和生理学过程提供了有力的观察手段,对研究细胞的功能以及疾病的发病机制有十分重要的影响,具有十分重要的生物医学意义。单分子定位成像技术作为超分辨成像技术的一个分支,具有重大的科研价值。从单分子定位成像技术的研究背景和意义出发,详细介绍了该技术的发展历程,对现有的主要单分子定位技术进行了较为详细的原理介绍和各自优缺点的分析,最后对单分子定位技术的实际应用进行了展望。
Optical super-resolution microscopy,enabling to break the diffraction limit,provides a powerful observation means for research on ultra-fine structures and physiological processes.It has an important impact on studying the functions of cells and the pathogenesis of diseases,which is of great biomedical significance.As a branch of super-resolution imaging methods,single molecule localization microscopy has great scientific research value.We first introduce the research background and significance of single molecule localization microscopy.Then we describe the development process of this technology in detail.In addition,the basic principles of the existing mainstream technologies are elaborated and their corresponding advantages and disadvantages are also analyzed.Finally,we prospect the applications of the single molecule localization technology.
作者
李雨竹
李传康
郝翔
刘旭
匡翠方
Li Yuzhu;Li Chuankang;Hao Xiang;Liu Xu;Kuang Cuifang(State Key Laboratory of Modern Optical Instrumentation,College of Optical Science and Engineering,Zhejiang University,Hangzhou,Zhejiang 310027,China;Ningbo Institute,Zhejiang University,Ningbo,Zhejiang 315100,China;Collaborative Innovation Center of Extreme Optics,Shanxi University,Taiyuan,Shanxi 030006,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2020年第24期19-34,共16页
Laser & Optoelectronics Progress
基金
国家自然科学基金重大科研仪器研制项目(61827825)
浙江省之江实验室项目(2020MC0AE01)。
关键词
医用光学
荧光成像
超分辨显微
单分子定位成像
光漂白
定位精度
medical optics
fluorescence microscopy
super-resolution microscopy
single molecule localization microscopy
photobleaching
localization accuracy
