摘要
目的研究过氧化物还原酶4(peroxiredoxin 4,PRDX4)在口腔鳞癌组织中的表达及临床意义。方法通过苏木素-伊红染色方法明确口腔鳞癌患者手术中所取组织的病理类型,荧光定量PCR实验检测30例口腔鳞癌患者正常组织和肿瘤组织中PRDX4的表达,通过免疫组织化学实验检测101例正常和口腔鳞癌组织中PRDX4的表达,并分析其与患者临床病理特征的关系。结果荧光定量PCR实验结果显示,PRDX4在正常组织中的2^(-△CT)值为2.064±0.2023,在口腔鳞癌组织中的2^(-△CT)值为4.170±0.2866(P<0.01)。免疫组织化学实验结果显示,PRDX4表达强度与口腔鳞癌患者生存率(P=0.0132)、淋巴结转移(P=0.0033)和组织病理分级(P=0.0353)显著相关。结论PRDX4在口腔鳞癌组织中高表达,且与患者不良预后存在密切关系。
Objective To investigate the expression and clinical significance of peroxiredoxin 4(PRDX4)in oral squamous cell carcinoma.Methods Hematoxylin-eosin was used to confirm the tissue histopathology of oral squamous cell carcinomas(OSCC)patients.Real-time PCR analysis was used to analyze the PRDX4 mRNA expression of 30 normal and OSCC tissues.Immunohistochemical assay was used to analyze the PRDX4 expression of 101 normal and OSCC tissues,as well as the relationship between PRDX4 expression and clinicopathological parameters,such as age,gender,histological grade and metastasis.Results Real time PCR showed that the relative PRDX4 expression in normal tissues was 2.064±0.2023,and 4.170±0.2866 in OSCC tissues(P<0.01).The immunohistochemistry results showed that higher PRDX4 levels were correlated with poor overall survival(P=0.0132),poor histological grade(P=0.0033)and nodal metastasis(P=0.0353)in OSCC.Conclusions PRDX4 was highly expressed in OSCC tissues,and higher PRDX4 expression was related with poor prognosis of OSCC patients.
作者
于玢玢
王丽珍
吴开柳
池政兵
严明
YU Bin-bin;WANG Li-zhen;WU Kai-liu;CHI Zheng-bing;YAN Ming(Shanghai Research Institute of Stomatology,Ninth People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China)
出处
《北京口腔医学》
CAS
2021年第1期7-12,共6页
Beijing Journal of Stomatology
基金
国家自然科学基金(81672829,81702979)。
关键词
口腔鳞癌
过氧化物还原酶4
免疫组织化学实验
预后
Oral squamous cell carcinomas
Peroxiredoxin 4
Immunohistochemical assay
Prognosis
