摘要
目的:探究美洲大蠊不同提取物的抗氧化活性。方法:用不同极性溶剂提取美洲大蠊提取物,制备得水提取物(PAW)、乙醇提取物(PAEE)、正丁醇提取物(PABA)、乙酸乙酯提取物(PAEA)和石油醚提取物(PAPE)。通过测定美洲大蠊不同提取物清除羟基、超氧阴离子、1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)4种自由基的能力,总还原能力和亚铁离子(Fe^(2+))螯合能力评估其抗氧化活性。结果:相同浓度下,PABA和PAEA对DPPH、ABTS自由基清除率以及总还原能力显著高于其他提取物,且PABA优于PAEA;PABA具有较强的Fe^(2+)螯合能力,PAEE次之;PAEE和PAW对羟基自由基和超氧阴离子自由基清除能力高于其他3种美洲大蠊提取物。结论:5种美洲大蠊提取物抗氧化活性存在明显的差异,但都具有一定的抗氧化活性,这为美洲大蠊资源的后续开发利用提供了实验依据。
Objective:To study the antioxidant activity of different extracts of Periplaneta americana.Methods:Extracts of Periplaneta americana were extracted with different polar solvents and prepared as water extract(PAW),ethanol extract(PAEE),nbutanol extract(PABA),ethyl acetate extract(PAEA)and petroleum ether extract(PAPE).The antioxidant activity of different extracts of Periplaneta americana was evaluated by determining the scavenging ability of hydroxyl radical,superoxide anion radical,DPPH radical,ABTS radical,total reduction ability and Fe^(2+)chelation ability.Results:At the same dose concentration,the scavenging rates of DPPH and ABTS and total reduction capacity were significantly higher at the PABA and PAEA than other extracts,and PABA was superior to PAEA.At the same time,the PABA had strong chelating ability of Fe^(2+)ion,followed by PAEE;PAEE and PAW had higher scavenging ability to free hydroxyl radicals and superoxide anions than the other three extracts.Conclusion:The above results showed that the antioxidant activity of 5 extracts of Periplaneta americana was significantly different,but all of them had certain antioxidant activity,which provided experimental basis for the further development and utilization of resources of Periplaneta americana.
作者
和英
顾婷
杨永寿
肖培云
He Ying;Gu Ting;Yang Yongshou;Xiao Peiyun(College of Pharmacy,Dali University,Dali,Yunnan 671000,China)
出处
《大理大学学报》
CAS
2021年第2期10-14,共5页
Journal of Dali University
基金
国家自然科学基金项目(81560634)
云南省地方本科高校(部分)基础研究联合专项资金项目(2019FH001-034)。
关键词
美洲大蠊提取物
抗氧化活性
自由基
总还原能力
Fe^(2+)螯合能力
extracts of Periplaneta americana
antioxidant activity
free radical
total reduction ability
Fe^(2+) chelation ability
