摘要
以48份石斛兰种质资源为对象,采用iPBS分子标记技术对其遗传多样性进行分析并构建DNA指纹图谱。结果表明:从83条iPBS引物中筛选出7条扩增条带清晰、多态性高、重复性好的引物;利用筛选出的引物对48份石斛兰基因组DNA进行PCR扩增,共获得279条谱带,其中多态性条带279条,多态性比例为100%;采用GenAlEx 6.5软件计算48份石斛兰的平均观测等位基因数(N_(a))为2.000,平均有效等位基因数(N_(e))为1.202,平均Nei’s遗传多样性指数(H_(e))为0.153,平均Shannon信息多样性指数(I)为0.274,48份石斛兰表现出丰富的遗传多样性;采用NTSYS-pc 2.1软件计算得到48份石斛兰间的遗传相似系数为0.6667~0.9211,基于遗传相似系数进行UPGMA聚类,在相似系数0.75处,可将48份石斛兰划分为7个类群。利用2对引物构建的DNA指纹图谱可单独鉴别出48份石斛兰种质资源,该图谱可为石斛兰分类与鉴定提供科学依据。
The genetic diversity of 48 Dendrobium germplasm resources was analyzed and DNA fingerprint was constructed by the iPBS molecular marker technique.The results showed that seven primers with clear amplification bands,high polymorphism and good repeatability were selected from 83 iPBS primers.The selected primers were used to amplify the genomic DNA of 48 Dendrobium germplasm resources by PCR,and a total of 279 bands were generated,of which 279 were polymorphic bands,and the polymorphic ratio was 100%.By GenAlEx 6.5 software,average value of observed allele number,effective number of alleles,Nei's gene diversity and Shannon's information index was 2.000,1.202,0.153 and 0.274,respectively,indicating that a high level of genetic diversity among the 48 Dendrobium germplasm resources.The genetic similarity coefficient among the 48 Dendrobium germplasm resources ranged from 0.6667 to 0.9211 by NTSYS-pc 2.1 software.UPGMA clustering based on genetic similarity coefficient revealed the 48 Dendrobium germplasm resources could be divided into seven groups when genetic similarity was 0.75.The DNA fingerprint map constructed with two pairs of primers could separately identify the 48 Dendrobium germplasm resources,which could provide a scientific basis for the classification and identification of Dendrobium.
作者
崔学强
唐璇
黄昌艳
邓杰玲
李秀玲
卢家仕
张自斌
CUI Xueqiang;TANG Xuan;HUANG Changyan;DENG Jieling;LI Xiuling;LU Jiashi;ZHANG Zibin(Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)
出处
《热带作物学报》
CSCD
北大核心
2021年第2期317-324,共8页
Chinese Journal of Tropical Crops
基金
广西创新驱动发展专项项目(桂科AA17204045-6,桂科AA17204026)
广西农业科学院基本科研业务专项项目(桂农科2020YM32)。
