摘要
目的:建立土茯苓配方颗粒水提液高效液相色谱特征图谱,为土茯苓配方颗粒的质量控制提供参考。方法:采用kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相:甲醇-0.05%磷酸水溶液,系统梯度洗脱,流速:1.0 mL/min,柱温:30℃,检测波长:280 nm。结果:对10批土茯苓配方颗粒进行测定,确定了土茯苓配方颗粒指纹图谱中14个共有峰,且10批样品的相似度在0.9以上。结论:该方法准确可靠,重复性好,可用于土茯苓配方颗粒的鉴别和质量评价。
Objective:To provide the reference for quality control of Tufuling(Smilacis Glabrae Rhizoma)dispensing granules by determining the fingerprint of Tufuling dispensing granules with HPLC.Methods:Kromasil C18 chromatographic column(4.6 mm×250 mm,5μm),mobile phase:methanol-0.05%phosphoric acid aqueous solution,systematic gradient elution,flow rate:1.0 mL/min,column temperature:30℃,detection wavelength:280nm.Results:After determining ten batches of Tufuling dispensing granules,14 common peaks were confirmed in the fingerprint of Tufuling dispensing granules,the similarity of ten batches of samples were higher than 0.9.Conclusion:The method,accurate,reliable and reproducible,could be used for quality assessment and identification of TuFuLing dispensing granules.
作者
张肖建
董璐萌
高晗
李军山
李振江
ZHANG Xiaojian;DONG Lumeng;GAO Han;LI Junshan;LI Zhenjiang(China Shineway Pharmaceutical Group Ltd.,Shijiazhuang 051430,China;Research Center for Hebei Provincial Herbal Dispensing Granules Engineering Technology)
出处
《西部中医药》
2021年第1期20-23,共4页
Western Journal of Traditional Chinese Medicine
基金
河北省科技计划项目(16272510,17272505D)。
