摘要
蛋白酶抑制剂(protease inhibitor,PI)在植物系统防御中发挥着关键作用。为研究番茄PI基因的表达模式及其编码蛋白的相关性质,本研究从番茄(Solanum lycopersicum)中克隆了一个PI基因家族的成员,命名为SLPI100(登录号:XP_004249682.1)。该基因编码区长为285 bp,编码94个氨基酸、蛋白的半衰期30 h、不稳定指数37.5、脂肪族指数121.17、平均疏水性0.189、理论等电点9.38、相对分子量10.52 kD、为疏水性蛋白。经同源性分析和多序列比对得知该基因具有PI功能结构域,属于马铃薯蛋白酶抑制剂I家族。qRT-PCR分析显示SLPI100在番茄根中的表达量最高,其次为叶,推测其在抗土传病害和叶部病害过程中发挥重要功能;在致病疫霉菌(Phytophthora infestans)的侵染下,该基因的相对表达量呈现先升高后下降的趋势,表明其在番茄与病原菌互作过程中发挥重要作用。本研究为番茄PI基因功能的鉴定以及培育抗病工程植株奠定了基础。
Protease inhibitor(PI)plays a key role in plant defense systems.In order to study the related properties and expression patterns of tomato PI gene,this study cloned a member of the PI gene family from tomato,named SLPI100(GenBank accession number:XP_004249682.1).The length of the coding region of this gene was 285 bp,with a predicted protein sequence of 94 amino acids,a half-life of 30 h,an instability index of 37.5,an aliphatic index of 121.17,an average hydrophobicity of 0.189,a theoretical isoelectric point of 9.38,and a relative molecular weight of 10.52 kD,a hydrophobic protein.The multi-sequence alignment and protein domain prediction indicated that the protein of this gene had a PI functional domain,belonged to the potato proteinase inhibitorⅠ(PinⅠ)family.qRT-PCR analysis showed that tomato SLPI100 had the highest expression in roots,followed by leaves,which was presumed to play an important role in the process of resistance to soil-borne diseases and leaf diseases;Under the induction of Phytophthora infestans,the relative expression of this gene increased at first and then decreased later,indicated that it played a role in the interaction between tomato and pathogens.This study laid the foundation for further identification of gene functions and cultivation of disease-resistant engineering plants.
作者
马宁
刘亚荣
崔军
洪雨慧
栾雨时
Ma Ning;Liu Yarong;Cui Jun;Hong Yuhui;Luan Yushi(School of Bioengineering,Dalian University of Technology,Dalian,116024)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第10期4656-4663,共8页
Genomics and Applied Biology
基金
国家自然科学基金项目(31872116)资助。
