摘要
目的探究妊娠期铅(Pb)、镉(Cd)暴露对新生大鼠海马脑源性神经营养因子(BDNF)及锌转移体7(ZnT7)蛋白表达的影响。方法妊娠SD大鼠分为4组:对照组(NC组,饮用蒸馏水)、Pb组(300 mg/L)、Cd组(10 mg/L)及Pb+Cd组(300 mg/L Pb+10 mg/L Cd),每组3只。采用饮水染毒,干预21 d。采集各组21 d龄新生大鼠的血液及海马组织样本,PE700Z型原子吸收光谱仪测定血液及海马组织Pb、Cd水平;水迷宫实验测定新生大鼠认知能力;蛋白免疫印迹(Western blot)法检测海马组织中BDNF及ZnT7蛋白水平;Pearson法分析Pb+Cd组新生大鼠海马组织中BDNF、ZnT7蛋白水平与血液、海马中Pb、Cd水平的相关性。结果与NC组比较,Pb组、Cd组及Pb+Cd组新生大鼠逃避潜伏期、进入盲端次数、海马组织中ZnT7蛋白水平显著增加,BDNF水平显著降低(P<0.05),其中以Pb+Cd组变化最明显。Pb+Cd组新生大鼠海马组织中BDNF水平与血液及海马组织Pb、Cd水平均呈负相关(P<0.05),ZnT7蛋白水平与血液及海马组织Pb、Cd水平均呈正相关(P<0.05)。结论妊娠期Pb、Cd暴露可协同降低新生大鼠认知能力,可能与海马BDNF表达降低,ZnT7蛋白表达升高有关。
Objective To explore the effect of plumbum(Pb)and cadmium(Cd)exposure during pregnancy on the expression of brain-derived neurotrophic factor(BDNF)and zinc transporter 7(ZnT7)protein in the hippocampus of newborn rats.Methods Pregnant SD rats were randomly divided into 4 groups:the control group(the NC group,drinking distilled water),the Pb group(300 mg/L of plumbum),the Cd group(10 mg/L of cadmium)and the Pb+Cd group(300 mg/L of plumbum+10 mg/L of cadium),with 3 animals in each group.Drinking water was used for poisoning,and the intervention time was 21 days.The blood and hippocampal tissue samples of newborn rats were collected from each group,and the levels of Pb and Cd in blood and hippocampus tissue were measured by PE700Z atomic absorption spectrometer;the cognitive ability of newborn rats was determined by water maze experiment;the expressions of BDNF and ZnT7 protein in hippocampus tissue were detected by Western blot.Pearson method was used to analyze the correlations between the levels of BDNF,ZnT7 proteins and the contents of plumbum,cadmium in blood and hippocampus tissue in the Pb+Cd group.Results Compared with NC group,the escape latency,times of entering blind end,the levels of ZnT7 protein in hippocampus of newborn rats in the Pb group,Cd group and Pb+Cd group were significantly higher,while the level of BDNF was significantly lower(P<0.05),and the changes in Pb+Cd group was the most obvious(P<0.05).The results of Pearson analysis showed that the level of BDNF in the hippocampus tissues of the Pb+Cd group was negatively correlated with the levels of blood and hippocampal plumbum and cadmium(P<0.05),and the level of ZnT7 protein was positively correlated with the levels of blood and hippocampus plumbum and cadmium(P<0.05).Conclusion Plumbum and cadmium exposure during pregnancy can synergistically reduce the cognitive ability of newborn rats,which may be related to the decrease of cadmium BDNF expression and the increase of ZnT7 protein expression in hippocampal.
作者
路艳清
孙蕾
王雪芳
LU Yanqing;SUN Lei;WANG Xuefang(Department of Obstetrics and Gynecology,Second People′s Hospital of Lanzhou City,Lanzhou,Gansu 730046,China;Department of Obstetrics and Gynecology,People′s Hospital of Wuwei City,Wuwei,Gansu 733000,China)
出处
《重庆医学》
CAS
2021年第5期737-740,745,共5页
Chongqing medicine
基金
2018年度兰州市科技发展指导性计划项目(2018-ZD-29)。
