摘要
番茄褪绿病毒(Tomato chlorosis virus),属于长线形病毒科(Closteroviridae)毛形病毒属(Crinivirus),主要由两条二分体线形正义单链RNA(+ssRNA)组成[1]。该病毒1998年在美国弗罗里达州首次报道[2]。迄今,ToCV已蔓延至我国山东、河南、河北、山西、陕西、浙江、内蒙古、广东、湖南等地,给我国蔬菜生产造成了严重损失[3]。
Tomato chlorosis virus(ToCV)is a plant virus that is mainly propagated by Bemisia tabaci in a semi-persistent and non-circulative manner.It has a wide range of host plants,and has been reported in many countries,causing serious economic losses in vegetable production.In 2019,we investigated about 10 fields,one ha each in Shouguang,Shandong province(China),and in each field we observed symptoms of interveinal chlorosis on lower leaves of the Solanum torvum Swartz,and a large number of B.tabaci gathered on the back of its leaves.To determine the presence of ToCV,total RNA of S.torvum was extracted followed by RT-PCR.The 1074(GenBank accessions number MN545620)and 466 bp(GenBank accessions number MN545621)fragments were gel purified and sequenced.The sequences shared 99.44%and 99.57%similarity with ToCV reference sequence tomato chlorosis virus segment RNA1(AY903447)and RNA2(AY903448).The results of insect transmission test confirmed that ToCV can spread from S.torvum to tomato.This study confirms S.torvum as a newly reported host of ToCV.
作者
廖锦钰
张战泓
张德咏
谭新球
史晓斌
刘勇
LIAO Jin-yu;ZHANG Zhan-hong;ZHANG De-yong;TAN Xin-qiu;SHI Xiao-bin;LIU Yong(Subcollege of Longping,Graduate School of Hunan University,Changsha 410128,China;Hunan Plant Protection Institute,Hunan Academy of Agricultural Science,Changsha 410125,China;Hunan Vegetable Institute,Hunan Academy of Agricultural Science,Changsha 410125,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2020年第6期789-792,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金资助项目(31872932、31672003)
湖南省自然科学基金资助项目(2019JJ30014)
大宗蔬菜产业体系(CARS-23-D-02)。
