摘要
为明确二斑叶螨对设施农业中温室高温环境胁迫的生理响应,在实验室内以二斑叶螨Tetrarcychus urticae雌成虫为试螨,设置36℃、39℃和42℃3个温度梯度,2 h、4 h和6 h 3个胁迫时间,测定各胁迫处理后其体内多功能氧化酶(MFO)、羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GST)的酶比活力以及酶动力学参数(米氏常数K m和最大反应速率V max)的变化。结果表明,经36℃,39℃和42℃胁迫后,二斑叶螨体内CarE、MFO、GST的酶比活力和酶动力学参数的变化均受到不同程度的影响。酶比活力:CarE的比活力均高于对照(除39℃胁迫6 h外),MFO的比活力也均高于对照(除42℃胁迫6 h外),GST的酶比活力均高于对照(除42℃胁迫2 h外)。经双因素方差分析,高温胁迫和处理时间之间存在显著的交互作用(P<0.05)。酶动力学参数:CarE的K m值均高于对照(除36℃胁迫2 h和4 h、39℃胁迫6 h外),MFO的K m值均低于对照(除39℃胁迫6 h、42℃胁迫4 h外),GST的K m值均高于对照(除36℃胁迫2 h外)。CarE的V max值均高于对照(除36℃胁迫2 h和4 h外),MFO的V max值均高于对照(除42℃胁迫4 h外),GST的V max值均高于对照。说明二斑叶螨可能通过调整体内解毒酶的酶比活力来应对高温胁迫,相比较CarE和GST,MFO与底物的亲和力最大,反应速率最快,表明其在应对高温胁迫的解毒响应中可能起主导作用。
In order to clarify the detoxification mechanisms of physiological response to heat stress on Tetrarcychus urticae(Koch)in greenhouse,the females adult of T.urticae were treated in 2 h,4 h,6 h under three different high temperatures(36℃,39℃and 42℃),respectively.The specific activity,enzyme kinetics(michaelis constants,K m and maximal velocities,V max)of multifunctional oxidase(MFO),carboxylesterase(CarE)and glutathione S-transferase(GST)of T.urticae were measured.The interaction between heat stress and treatment time showed a significant difference tested by two-way ANOVA(P<0.05).The results showed that,the specific activity and enzyme kinetics of CarE,MFO,GST were differently affected.Specific activity:the specific activity of CarE was higher than the control except the 6 h treatment at 39℃,the specific activity of MFO was higher than the control except the 6 h treatment at 42℃,the specific activity of GST was higher than the control except the 2 h treatment at 42℃.Enzyme kinetics:the K m value of CarE was higher than the control except the 2 h,4 h treatment at 36℃and 6 h treatment at 39℃,the K m value of MFO was lower than the control except the 6 h treatment at 39℃and 4 h treatment at 42℃,the K m value of GST was higher than the control except the 2 h treatment at 36℃.The V max value of CarE was higher than the control except the 2 h,4 h treatment at 36℃,the V max value of MFO was higher than the control except the 4 h treatment at 42℃,the V max value of GST was higher than the control in all treatments.The results indicated that T.urticae can change the specific activity of detoxification enzymes to resist high temperature.MFO had the highest affinity with substrate and the fastest catalytic rate,it was likely to play a dominant role in the response of resisting high temperature,compared with CarE and GST.
作者
王常清
韩永金
曹进刚
张洁
尚素琴
WANG Chang-Qing;HAN Yong-Jin;CAO Jin-Gang;ZHANG Jie;SHANG Su-Qin(College of Plant of Gansu Agricultural University,Biological Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China)
出处
《环境昆虫学报》
CSCD
北大核心
2021年第1期181-190,共10页
Journal of Environmental Entomology
基金
甘肃农业大学青年导师基金(GAU-QNDS-201606)
甘肃农业大学研究生重点课程建设项目(GSAU-ZDKC-2016)。
关键词
二斑叶螨
高温胁迫
解毒酶
酶动力学参数
Tetrarcychus urticae
high temperature
detoxification enzymes
enzyme kinetics
