摘要
目的:研究风湿热应激前后雷公藤配伍金钱草抗炎增效及减毒作用。方法:90只SPF级昆明小鼠随机分为正常对照组、角叉菜胶组、雷公藤组、金钱草组、雷公藤配伍金钱草组(配伍组)、单纯应激组、雷公藤应激组、金钱草应激组、配伍应激组。雷公藤组、金钱草组、配伍组分别以2、1、3g/kg的剂量灌胃给药14d,1次/d。通过角叉菜胶炎症模型评价雷公藤、金钱草、雷公藤配伍金钱草在两种状态下的抗炎作用,通过检测小鼠血清相关指标(AST、ALT、BUN、Cr)和肝、肾脂质过氧化指标(T-SOD、MDA),探讨金钱草在两种状态下对雷公藤的减毒作用。结果:雷公藤组、金钱草组、配伍组在风湿热应激前后均使小鼠在角叉菜胶致炎30~90min后足肿胀度显著降低(P<0.05,P<0.01),且配伍组足肿胀度在各自条件下最低,且配伍应激组相对于雷公藤应激组的足肿胀抑制率提高幅度小于配伍组相对于雷公藤组。雷公藤组在风湿热应激前后均使小鼠血清AST、ALT、BUN、Cr水平升高和肝肾T-SOD、MDA水平降低(P<0.01),而配伍组在风湿热应激前后均可逆转这种改变(P<0.05,P<0.01),雷公藤应激组相对于单纯应激组血清和肝肾中各指标的变化率大于雷公藤组相对于角叉菜胶组,而配伍应激组相对于单纯应激组的变化率小于配伍组相对于角叉菜胶组。结论:风湿热应激前后雷公藤配伍金钱草皆具有抗炎增效减毒的作用,其中应激后抗炎增效作用减弱,而减毒作用增强,其配伍减毒体现了中医中"有故无殒"思想,减毒机制可能与抑制肝脂质过氧化并增强抗氧化水平有关。
Objective:To study the anti-inflammatory synergism and attenuated virulence effects of tripterygium wilfordii radix(LGT)combination with christina loosestrife(JQC)before and after rheumatic heat stress.Methods:Ninety SPF Kunming mice were randomly divided into normal control group,Carrageenan group,LGT,JQC and LGT combined with JQC(LGTJQC)group,rheumatic heat stress group(RFS),RFS+LGT group,RFS+JQC group,RFS+LGT-JQC group.LGT,JQC and LGTJQC were administered by gavage at the doses of 2,1,3 g/kg for 14 days,once a day.The anti-inflammatory effects of LGT,JQC and LGT combined with JQC were evaluated by carrageenan inflammation model.The attenuation effects of JQC combined with LGT were studied by detecting the levels of serum related indexes(AST,ALT,BUN,Cr)and liver and kidney lipid peroxidation indexes(T-SOD,MDA).Results:LGT,JQC and LGT combined with JQC significantly reduced the foot swelling of mice after 30~90 minutes of carrageenan-induced inflammation(P<0.05,P<0.01),and the foot swelling of the compatibility group was the lowest under their respective conditions.And the increase of the foot swelling inhibition rate of the RFS+LGT-JQC group relative to the RFS+LGT group was less than that of the LGT-JQC group relative to the LGT group.LGT could increase the levels of AST,ALT,BUN and Cr in serum and decrease the levels of T-SOD and MDA in liver and kidney of mice before and after winddampness-heat stress(P<0.01).The combination of LGT and JQC could reverse the changes before and after wind-dampnessheat stress(P<0.05,P<0.01).RFS+LGT group relative to RFS group serum and liver and kidney indicators of the change rate was greater than LGT group relative to carrageenan group,while RFS+LGT-JQC group relative to RFS group change rate was less than LGT-JQC group relative to Carrageenan group.Conclusion:The compatibility of LGT with JQC before and after rheumatic heat stress has the effect of anti-inflammatory synergism and attenuation,and the stress weakens the anti-inflammatory synergism of LGT with JQC and enhances the attenuation effect.Its compatibility attenuation embodies the thought of‘no cause,no loss’in traditional Chinese medicine.The mechanism of attenuation may be related to inhibiting liver lipid peroxidation and enhancing antioxidant levels.
作者
巫晓慧
王君明
宋玲玲
关月晨
刘晨
曹灿
WU Xiao-hui;WANG Jun-ming;SONG Ling-ling;GUAN Yue-chen;LIU Chen;CAO Can(College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment&Chinese Medicine Development of Henan Province,Henan University of Chinese Medicine,Zhengzhou 450046,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2021年第2期1006-1011,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金青年科学基金项目(No.81503269)
河南中医药大学科技创新人才支持计划项目(No.2015XCXRC01)。
关键词
风湿热应激
角叉菜胶
炎症
增效减毒
有故无殒
配伍
Rheumatic fever stress
Carrageenan
Inflammation
Synergism and attenuation
No cause
no loss
Synergy
