摘要
目的:分析中性粒细胞表面分化抗原CD67、CD24在阵发性睡眠性血红蛋白尿症(PNH)表达水平及临床意义。方法:回顾性分析我院32例PNH患者(PNH组)临床资料,并收集同期入院治疗的28例再生障碍性贫血(AA)患者(AA组)及32例健康体检者(对照组)临床资料。比较3组中性粒细胞表面分化抗原CD67、CD24、CD55、CD59水平差异,评估PNH组上述指标表达水平的相关性,并使用受试者工作曲线(ROC曲线)评估上述中性粒细胞表面分化抗原对PNH的诊断价值。结果:3组性别、年龄、体质量指数、吸烟史、饮酒史比较,差异均无统计学意义(P>0.05)。3组中性粒细胞表面CD67、CD24、CD55、CD59表达水平比较,均为PNH组<AA组<对照组(P<0.05);经Pearson相关性分析,发现PNH组中性粒细胞表面CD67、CD24、CD55、CD59表达水平均呈显著正相关(P<0.05)。经ROC曲线分析,发现中性粒细胞表面CD67、CD24、CD55、CD59表达水平均对PNH具有较高诊断价值(AUC=0.961、0.967、0.972、0.995,P<0.05),其cut-off值分别为81.88%、83.64%、84.42%、81.37%,且4项联合检测诊断价值最高(AUC=1.000,P<0.05)。结论:中性粒细胞表面分化抗原CD67、CD24表达水平在PNH中严重降低,与CD55、CD59表达水平变化情况一致,4项指标联合检测诊断PNH价值较高,对临床诊疗有指导作用。
Objective:To analyze the expression levels and clinical significance of neutrophil surface differentiation antigens CD67 and CD24 in paroxysmal nocturnal hemoglobinuria(PNH).Methods:The clinical data of 32 patients with PNH(PNH group)in our hospital were retrospectively analyzed,and the clinical data of 28 patients with aplastic anemia(AA)(AA group)and 32 healthy subjects with physical examination(control group)admitted to the hospital during the same period were collected.The differences in the levels of neutrophil surface differentiation antigens CD67,CD24,CD55 and CD59 were compared among the three groups.The correlation among the expression levels of the above indicators was evaluated in PNH group,and receiver operating characteristic curves(ROC curves)were used to evaluate the diagnostic value of neutrophil surface differentiation antigens on PNH.Results:There were no statistically significant differences in gender,age,body mass index,smoking history and drinking history among the three groups(P>0.05).Comparison of expression levels of neutrophil surface CD67,CD24,CD55 and CD59 in the three groups showed that PNH grouP<AA grouP<control group(P<0.05).Pearson correlation analysis showed that expression levels of neutrophil surface CD67,CD24,CD55 and CD59 were significantly positively correlated with each other in PNH group(P<0.05).After ROC curve analysis,it is found that the expression levels of neutrophil surface CD67,CD24,CD55 and CD59 had high diagnostic value on PNH(AUC=0.961,0.967,0.972,0.995,P<0.05),and their cut-off values were 81.88%,83.64%,84.42%and 81.37%respectively,and the combination of the four had the highest diagnostic value(AUC=1.000,P<0.05).Conclusion:The expression levels of neutrophil surface differentiation antigens CD67 and CD24 are severely reduced in PNH,and they are consistent with the changes of expression levels of CD55 and CD59,and the combined detection of the four indicators is of high value in diagnosing PNH,and can guide clinical diagnosis and treatment.
作者
丁园
熊涛
DING Yuan;XIONG Tao(Hanchuan People's Hospital,Hubei Xiaogan 431600,China)
出处
《河北医学》
CAS
2021年第3期476-480,共5页
Hebei Medicine
基金
湖北省卫生计生委2018年度联合基金立项项目,(编号:WJ2018H0087)。
