摘要
试验研究不同条件对植酸酶水浴耐热性的影响。分别稀释4种植酸酶酶活力40、20、10、2 U/mL,测定80℃和85℃水浴5 min的耐热性。结果表明,稳定性强的植酸酶受酶浓度的影响较小,稳定性较差的植酸酶受影响较大,当处理液酶活力低时,耐热性结果较高。取3种植酸酶样品,分别使用缓冲液Ⅰ、缓冲液Ⅱ、水为耐温稀释缓冲液,测定85℃水浴5 min耐热性,结果表明:缓冲液Ⅰ作为稀释缓冲液测定的耐热性结果最高,其次是缓冲液Ⅱ,水作为稀释缓冲液时测定的耐热性结果最低。取3种植酸酶样品,分别在pH值5.5和5.0缓冲体系中测定85℃水浴5 min耐热性,结果表明,植酸酶在与最适pH值相近的条件下进行耐热性处理,测定的耐热性结果相对较高。研究表明,本试验的3种条件对水浴法测定植酸酶耐热性的结果均有影响,制定检测标准时需要明确测定条件。
The effect of different conditions on the temperature-resistance of phytase by water-bath method were studied.Four samples were diluted to 40, 20, 10, 2 U/mL separately. The testing temperature was set at 80 ℃, 85 ℃ water bath for5 min. The results showed that the phytase with strong stability was less affected by the enzyme concentration, and the phytase with poor stability was more affected. When the enzyme activity of the treatment solution was low, the heat resistance results were higher. Take three phytase samples, use buffer I, buffer II, and water as the temperature-resistant dilution buffer respectively, and measure the heat resistance in a water bath at 85 ℃ for 5 min. The results showed that the heat resistance of buffer I was the highest as the dilution buffer, followed by buffer II, and the heat resistance was the lowest when water was used as the dilution buffer. Take three samples of phytase and measure the heat resistance in a water bath at 85 ℃ for 5 min in p H 5.5 and 5.0 buffer systems. The results showed that phytase was subjected to heat resistance treatment under conditions similar to the optimum p H value. The heat resistance results were relatively high. Therefore, the three conditions tested in this research all have an impact on the results of measuring the heat resistance of phytase by the water bath method, and the determination conditions need to be clarified when formulating the test standards.
作者
邵静
于靖
王蕊
张帅
王华明
Shao Jing;Yu Jing;Wang Rui;Zhang Shuai;Wang Huaming(Weifang KDN Biotech Co.,Ltd.Qingdao Branch,Shandong Qingdao 266100;Weifang KDN Biotech Co.,Ltd.,Shandong Weifang 261500;Qingdao Vland Biotech Co.,Ltd.,Shandong Qingdao 266100)
出处
《现代畜牧兽医》
2021年第3期10-13,共4页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
科技部国家重点研发计划“政府间国际科技创新合作”专项(2016YFE0113900)。
关键词
水浴法
植酸酶耐热性评价
酶浓度
缓冲体系
PH值
Water-bath method
Evaluation of temperature-resistant for phytase
Enzyme concentration
Buffer system
pH value
