摘要
目的分析宫颈鳞癌中血管内皮生长因子(vascular endothelial growth factor,VEGF)-C、VEGF受体(VEGF receptor,VEGFR)-3的表达与人乳头瘤病毒(human papillomavirus,HPV)16/18感染的相关性。方法收集2017年3月至2019年3月德阳市人民医院收治的147例宫颈病变患者为研究对象,其中包括宫颈上皮内瘤变(cervical intraepilthelial neoplasias,CIN)I样本25例、CIN II样本15例、CIN III样本32例、宫颈鳞癌样本75例。另外收集同期体检的健康女性正常样本18例。采用原位杂交法分析其HPV16及HPV18感染情况,采用免疫组化分析VEGF-C、VEGFR-3表达水平及其相关性。结果正常宫颈样本的HPV16、HPV18及HPV16、18双重感染率与CIN I、CIN II、CIN III及宫颈鳞癌相比,差异有统计学意义(P<0.05);CIN I、CIN II、CIN III的HPV16、HPV18及HPV16、18双重感染率与及宫颈鳞癌相比,差异无统计学意义(P>0.05)。免疫组化结果显示,正常宫颈样本、CIN I、CIN II和CIN III病理组织中的VEGF-C表达和VEGFR-3脉管阳性均数均低于宫颈鳞癌组,其中正常宫颈样本中的VEGF-C表达和VEGFR-3脉管阳性均数均低于CIN I、CIN II和CIN III;CIN I病理组织中的VEGF-C表达和VEGFR-3脉管阳性均数均低于CIN III,差异有统计学意义(P<0.05);CIN I病理组织中的VEGF-C表达和VEGFR-3脉管阳性均数与CIN II比较,CIN II病理组织中的VEGF-C表达和VEGFR-3脉管阳性均数与CIN III比较,差异均无统计学意义(P>0.05)。75例宫颈鳞癌组织中,HPV16/18阳性组VEGF-C表达明显高于HPV16/18阴性组(P<0.05)。经Spearman相关性分析,VEGF-C表达水平与VEGFR-3脉管阳性均数呈正相关(P<0.05)。结论宫颈鳞癌发生与HPV16/18感染相关,VEGF-C、VEGFR-3的高表达可一定程度反映宫颈病变严重程度。
Objective To analyze the correlation between the expression of vascular endothelial growth factor(VEGF)-C and VEGF receptor(VEGFR)-3 and human papillomavirus(HPV)16/18 infection in cervical squamous cell carcinoma.Methods 147 patients with cervical lesions admitted to Deyang People's Hospital from March 2017 to March 2019 were collected as the research objects,including 25 cervical intraepilthelial neoplasias(CIN)I samples,15 CIN II samples,32 CIN III samples and 75 samples of cervical squamous cell carcinoma.In addition,18 normal samples of healthy women who received physical examination during the same period were collected.In situ hybridization was used to analyze the infection of HPV16 and HPV18,and immunohistochemistry was used to analyze the expression levels of VEGF-C and VEGFR-3 and their correlation.Results Compared with CIN I,CIN II,CIN III and cervical squamous cell carcinoma,the infection rates of HPV16,HPV18 and dual infection rate in normal cervical samples were significantly different(P<0.05);compared with cervical squamous cell carcinoma,the infection rates of HPV16,HPV18 and dual infection rate of CIN I,CIN II,and CIN III were not statistically different(P>0.05).The results of immunohistochemistry showed that the expression of VEGF-C in normal cervical samples,CIN I,CIN II,and CIN III pathological tissues and the positive mean of VEGFR-3 vessels were lower than those in the cervical squamous cell carcinoma group.Among them,the expression of VEGF-C and the mean number of positive VEGFR-3 vessels in normal cervical samples were lower than CIN I,CIN II and CIN III;the expression of VEGF-C and VEGFR-3 in the pathological tissues of CIN I were lower than those of CIN III,the difference was statistically significant(P<0.05);Compared with CIN II,the expression of VEGF-C and the mean number of positive VEGFR-3 vessels in the pathological tissue of CIN I were not significantly different,so was CIN II compared with CIN III(P>0.05).In 75 cases of cervical squamous cell carcinoma,the expression of VEGF-C in HPV16/18 positive group was significantly higher than that in HPV16/18 negative group(P<0.05).The Spearman correlation analysis showed that the expression level of VEGF-C was positively correlated with the mean number of positive VEGFR-3 vessels(P<0.05).Conclusion The occurrence of cervical squamous cell carcinoma is related to HPV16/18 infection.The high expression of VEGF-C and VEGFR-3 can reflect the severity of cervical lesions to a certain extent.
作者
徐志红
王秀
刘小燕
刘洋
周萍
XU Zhihong;WANG Xiu;LIU Xiaoyan;LIU Yang;ZHOU Ping(Department of Reproductive Genetics,Deyang People's Hospital,Deyang Sichuan 618000,P.R.China;Department of Gynecology,Deyang People's Hospital,Deyang Sichuan 618000,P.R.China)
出处
《中国计划生育和妇产科》
2021年第3期56-59,73,I0002,共6页
Chinese Journal of Family Planning & Gynecotokology
基金
四川省卫健委科研课题(项目编号:130439)。
