摘要
虫生真菌的毒力退化是一种普遍现象,表现为产孢能力下降或丧失、毒力下降及次级代谢产物合成能力下降等。基于玫烟色棒束孢Isaria fumosorosea PF904菌株继代培养后产孢量和毒力严重退化,本试验采用添加4种不同营养物质的培养基对其进行复壮。结果表明:培养基中添加甲壳素粉后菌株毒力恢复最好,复壮第10代时对小菜蛾幼虫的致死中时(LT_(50))由初始菌株的5.86 d下降至3.32 d,接菌第7天校正死亡率高于82%,且恢复比例较稳定;蚕蛹培养基复壮后菌株产孢量恢复最好,复壮第10代时,产孢量由初始菌株的4.28×10^(6)个/cm^(2)提升至1.73×10^(7)个/cm^(2),提升3倍之多,但毒力恢复较差;而蝉蜕培养基复壮后产孢量和毒力恢复均不稳定;添加3种营养物质的综合培养基,第10代LT_(50)和产孢量分别恢复为3.53 d和1.58×10^(7)个/cm^(2),对两者具有较好的恢复效果。
Entomogenous fungal degradation was a common phenomenon,which is manifested by reduced or lost conidial production capacity,decreased virulence,and reduced secondary metabolite synthesis ability,etc.Isaria fumosorosea after subculture severely degraded in conidial production and virulence.In this study,the medium was supplemented with 4 different nutrients.The results showed that the virulence recovery of the strain was the best after rejuvenation with chitin medium.At the 10th generation,the LT_(50) decreased from 5.86 d of the original strain to 3.32 d.The Plutella xylostella larvae had a corrected mortality higher than 82% on the 7th day,and the recovery ratio was stable;the conidial production of the strain recovered best after rejuvenation with the silkworm chrysalis medium,increasing from 4.28×10^(6) conidia/cm^(2) of the original strain to 1.73×10^(7) conidia/cm^(2),which increased by three times,but the recovery of virulence was poor;the recovery of two indicators after the rejuvenation with cicada medium was unstable.The comprehensive medium added with three kinds of nutrients had a good recovery effect on both virulence and conidia production,and the 10th generation LT_(50) was 3.53 d and conidial production was 1.58×10^(7) conidia/cm^(2).
作者
郭恒
赵义涛
郑海霞
张仙红
GUO Heng;ZHAO Yitao;ZHENG Haixia;ZHANG Xianhong(College of Agronomy,Shanxi Agricultural University,Taigu 030801,China)
出处
《植物保护》
CAS
CSCD
北大核心
2021年第2期150-155,共6页
Plant Protection
基金
山西省自然科学基金(201801D121253)
山西省重点研发计划(201903D221021)。
关键词
玫烟色棒束孢
复壮
毒力
产孢量
Isaria fumosorosea
rejuvenation
virulence
conidia production
