摘要
男性不育是影响生殖健康的重要问题,由于致病因素和发病机制复杂,临床上针对非梗阻性无精子症等不明原因的男性不育症往往缺乏特异性治疗措施。随着生殖细胞体外诱导培养的技术的发展,研究者们希望利用无精子症患者源性的多能干细胞体外培养出自身具有功能性的精子,从而治疗男性不育症。体外培养生殖细胞的策略,通常是先将诱导多能干细胞分化为原始生殖样细胞,然后进一步诱导分化为单倍体精子样细胞,其培养的难点在于将二倍体生殖细胞减数分裂为单倍体的精细胞,但是目前这个问题还没有得到解决。我们对诱导多能干细胞诱导雄性配子的技术进展和原理进行综述,希望能帮助我们更好地理解体外精子发生的机制,为体外受精的临床应用提供参考。
Male infertility is an important issue affecting reproductive health,and its etiology and mechanism are complex or not clear.For patients with azoospermia,specific clinical treatments are limited.Therefore,to generate patients'own functional sperms is absolutely necessary.With the development of techniques,the scientists start to think about how to attain functional sperms deriving from human induced pluripotent stem cells(iPSCs)in vitro.Mainly,sperm induction with iPSCs includes two steps,first,the formation of diploid primordial germ cell,then the differentiation of haploid mature sperms.Technically,the difficulties lie in the generation of meiosis from a diploid cell into haploid sperm,and unfortunately,the problem is not solved yet.In order to understand this kind of knowledge well,we try to review the technical progress and principle of male gamete induction from iPSCs.Hopefully,it can help us to understand the mechanism of spermatogenesis in vitro well,and to provide a reference for the clinical application of in vitro fertilization.
作者
毋姗姗
范文娟
李瑞玲
邓锦波
Wu Shanshan;Fan Wenjuan;Li Ruiling;Deng Jinbo(School of Nursing and Health,Henan University,Kaifeng 475000,China;National Health Commission Key Laboratory of Birth Defects Prevention,Henan Key Laboratory of Population Defects Prevention,Henan Institute of Reproduction Health Science and Technology,Zhengzhou 450002,China)
出处
《中华生殖与避孕杂志》
CSCD
北大核心
2021年第1期89-94,共6页
Chinese Journal of Reproduction and Contraception
基金
河南省科技厅科技攻关项目(192102310134)
国家卫健委出生缺陷预防重点实验室开放课题(ZD201903)。
关键词
多能干细胞
雄性生殖细胞
精子发生
诱导分化
Pluripotent stem cells
Male germ cells
Spermatogenesis
Differentiation in vitro
