摘要
目的建立测定寒湿郁肺汤中11个主成分含量的方法。方法采用超高效液相色谱串联质谱法(UHPLCMS/MS),色谱柱为Agilent ZORBAX SB-C18(3.5μm,2.1 mm×150 mm),流动相为0.2%甲酸-10 mmol/L醋酸铵水溶液(A)-乙腈(B),梯度洗脱(0~0.6 min, 80%~40%A;0.6~1 min, 40%~30%A;1~4.3 min, 30%~5%A),流速0.3 ml/min,柱温40℃。以利血平为检测内标,采用多反应监测模式同时测定香草酸、厚朴酚、和厚朴酚、汉黄芩素、刺槐素、6-姜酚、桔皮素、羌活醇、川陈皮素、紫花前胡苷、橙皮苷11个化学主成分的含量。结果寒湿郁肺汤中11个主成分在各自浓度范围内线性关系良好(r>0.98),平均回收率在93.11%~111.73%。结论该实验所建立的方法操作简便、重复性好,为寒湿郁肺汤的质量控制提供了实验室依据。
Objective To establish a method to determine 11 main components in Hanshi yufei decoction.Methods The method adopted UHPLC-MS/MS with an Agilent ZORBAX SB-C18(3.5μm,2.1 mm×150 mm)column.The mobile phase was consisted of 0.2%formic acid plus 10 mmol/L ammonium acetate aqueous solution(A)-acetonitrile(B)and gradient elution(0–0.6 min,80%–40%A;0.6–1 min,40%–30%A;1–4.3 min,30%–5%A)at 0.3 ml/min.The column temperature was 40℃and 11 main components including vanillic acid,magnolol,honokiol,wogonin,sophorin,6-gingerol,citrinin,qianghuo alcohol,nobiletin,nodakenin,and hesperidin were quantified in a multiple reaction monitoring mode.The reserpine was the standard.Results The11 main components in Hanshi Yufei decoction had a good linear relationship within their concentration range(r>0.98),and the average recovery was 93.11%~111.73%.Conclusion The UHPLC-MS/MS method established in this experiment is easy to operate and has good reproducibility,which provides a laboratory basis for the quality control of Hanshi Yufei decoction.
作者
崔莉莉
陈勇
张蒙伟
鄢红霞
高守红
陈万生
CUI Lili;CHEN Yong;ZHANG Mengwei;YAN Hongxia;GAO Shouhong;CHEN Wansheng(Department of Pharmacy,Changzheng Hospital Affiliated to Navel Medical University,Shanghai 200003,China;Yichun University,Yichun 336000,China;Yunnan University of Chinese Medicine,Kunming 650000,China)
出处
《药学实践杂志》
CAS
2021年第2期148-151,156,共5页
Journal of Pharmaceutical Practice
基金
上海市临床药学重点专科建设项目(2016-40044-002)
上海市卫生计生系统重要薄弱学科建设项目(2016ZB0303)。
