摘要
目的探讨溃疡性结肠炎(UC)患者结肠组织中维生素D受体(VDR)表达水平与Th17/调节性T细胞(Treg)比例失衡的关系。方法回顾性收集30例UC患者和15例结肠腺瘤对照者的外周血和结肠组织标本,同时记录上述研究对象的临床资料。采用电化学发光法检测血清25-羟维生素D[25(OH)D]水平;采用免疫组化检测结肠组织中VDR、Th17细胞和Treg细胞相关因子[RORγt、白介素(IL)-17、Foxp3、IL-10]的表达水平。结果UC患者结肠组织中RORγt、IL-17、Foxp3、IL-10表达水平及RORγt/Foxp3比值均高于对照组[0.32(0.20,0.46)×10^(-2)比0.17(0.11,0.19)×10^(-2),0.56(0.30,0.76)×10^(-2)比0.19(0.16,0.26)×10^(-2),0.26(0.16,0.33)×10^(-2)比0.18(0.12,0.21)×10^(-2),0.29(0.19,0.37)×10^(-2)比0.06(0.04,0.09)×10^(-2),1.14(0.81,1.86)比0.86(0.78,0.92),P均<0.05],并且活动期患者高于缓解期患者[0.45(0.31,0.53)×10^(-2)比0.16(0.13,0.20)×10^(-2),0.73(0.56,0.78)×10^(-2)比0.26(0.21,0.33)×10^(-2),0.26(0.24,0.35)×10^(-2)比0.17(0.14,0.26)×10^(-2),0.35(0.28,0.38)×10^(-2)比0.16(0.11,0.20)×10^(-2),1.77(0.90,2.07)比82.84(64.12,112.67),P均<0.05]。UC患者结肠组织中VDR表达水平显著低于对照组[0.66(0.28,19.15)×10^(-2)比2.94(2.61,3.21)×10^(-2),P<0.001],并且活动期患者低于缓解期患者[0.37(0.26,0.69)×10^(-2)比2.63(1.78,3.24)×10^(-2),P<0.001]。线性回归分析发现UC患者结肠组织中RORγt/Foxp3比值与VDR表达水平成独立负相关(β=-0.530,P=0.003)。结论UC患者结肠组织中VDR表达降低与Th17/Treg细胞失衡相关。
Objects To explore the association of colonic vitamin D receptor(VDR)expressionwith imbalance of Th17/regulatory T cells(Tregs)in patients with ulcerative colitis(UC).Methods The specimens of peripheral blood and colonic tissue were retrospectively collected from 30 UC patients and 15 controls(colonic polypadenoma),respectively.The clinical data of all the participants were simultaneously recorded,including age,gender,smoking,body mass index,UC activity(assessed by Mayo scoring system).The electrochemiluminescence method was conducted to measure the serum level of 25-hydroxyvitamin D[25(OH)D].Moreover,the immunohistochemical staining method was applied for examining the protein expression levels of VDR,Th17 and Treg cells related factors(RORγt,IL-17,Foxp3,IL-10,respectively)in the colonic tissues.Results Compared with the controls,the expressions of RORγt,IL-17,Foxp3 and IL-10,together with the ratio of RORγt/Foxp3,were significantly higher in UC patients[0.32(0.20,0.46)×10^(-2)vs.0.17(0.11,0.19)×10^(-2),0.56(0.30,0.76)×10^(-2)vs.0.19(0.16,0.26)×10^(-2),0.26(0.16,0.33)×10^(-2)vs.0.18(0.12,0.21)×10^(-2),0.29(0.19,0.37)×10^(-2)vs.0.06(0.04,0.09)×10^(-2),1.14(0.81,1.86)vs.0.86(0.78,0.92),all P<0.05],meanwhile the expressions of RORγt,IL-17,Foxp3,IL-10,and the ratio of RORγt/Foxp3 were higher in patients with active UC than inactive UC[0.45(0.31,0.53)×10^(-2)vs.0.16(0.13,0.20)×10^(-2),0.73(0.56,0.78)×10^(-2)vs.0.26(0.21,0.33)×10^(-2),0.26(0.24,0.35)×10^(-2)vs.0.17(0.14,0.26)×10^(-2),0.35(0.28,0.38)×10^(-2)vs.0.16(0.11,0.20)×10^(-2),1.77(0.90,2.07)vs.82.84(64.12,112.67),all P<0.05].The colonic VDR expression in UC patients was significantly lower than that in controls[0.66(0.28,19.15)×10^(-2)vs.2.94(2.61,3.21)×10^(-2),P<0.001],meanwhile VDR expression was also shown to be lower in patients with active UC than that in those with inactive UC[0.37(0.26,0.69)×10^(-2)vs.2.63(1.78,3.24)×10^(-2),P<0.001].The subsequent linear regression analysis suggested that the ratio of RORγt/Foxp3 in colonic tissue of UC patients was independently and negatively associated to the colonic VDR expression(β=-0.530,P=0.003).Conclusion The lower colonic VDR expression is associated to the imbalance of Th17/Treg cells in UC patients.
作者
夏盛隆
闵全佳
邵晓晓
曹曙光
林道泼
吴利敏
胡定元
全多多
蒋益
Xia Shenglong;Min Quanjia;Shao Xiaoxiao;Cao Shuguang;Lin Daopo;Wu Limin;Hu Dingyuan;Quan Duoduo;Jiang Yi(Department of Gastroenterology,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou 325000,Zhejiang Province,China;Department of Pathology,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou 325000,Zhejiang Province,China)
出处
《中华炎性肠病杂志（中英文）》
2021年第1期62-67,共6页
Chinese Journal of Inflammatory Bowel Diseases
基金
浙江省自然科学基金(LY17H030011)
浙江省中医药科学研究基金(2019ZB075)
温州市科技局项目(Y2020011)。
