摘要
通过微波消解法,原子吸收分光光度计对当归中铅的吸光度进行测定,采用Box-Behnken Design设计试验方法以确定最佳仪器分析条件.通过单因素试验,选取灰化温度、灰化时间、原子化温度、原子化时间4因素进行3水平试验,通过Box-Behnken Design建立数学模型,方差分析、响应曲面分析、愿望函数优化寻求最佳条件.结果显示建立的数学模型R^(2)=0.9671,R^(2)_(Adj)=0.9342说明该模型具有很好的可信度,可用此模型进行预测,变异系数CV%=3.62%,表明在该试验条件下,变异程度较小,该模型具有较好的重复性,当灰化温度为525℃、灰化时间为20 s、原子化温度为1550℃、原子化时间为5 s时吸光度最大为0.24.因此,本研究可为原子吸收分光光度计测定铅元素提供很好的理论基础.
The absorbance of lead in flour was determined by microwave digestion method and atomic absorption spectrophotometer.Box-behnken Design was used to determine the best instrumental analysis conditions.Through the single-factor test,the four factors of ashing temperature,ashing time,atomization temperature and atomization time were selected for the three-level test.The mathematical model was established by Box-Behnken Design,and the optimal conditions were sought by variance analysis,response surface analysis and desire function optimization.Results show that the established mathematical model of the R^(2)=0.9671,R^(2)_(Adj)=0.9342 shows that the model has good credibility,this model could be used to predict the coefficient of variation CV%=3.62%,indicates that under the condition of the experiment,variation is small,the model has good repeatability,when ashing temperature is 525℃,ashing time is 20 s,atomization temperature is 1550℃,atomization time for 5 s absorbance maximum 0.24;Therefore,this study can provide a good theoretical basis for the determination of lead by atomic absorption spectrophotometer.
作者
王昭国
WANG Zhaoguo(Food Inspection and Testing Center,Zhangye Quality Inspection and Testing Institute,Zhangye 734000,China)
出处
《吉林化工学院学报》
CAS
2021年第3期7-12,共6页
Journal of Jilin Institute of Chemical Technology
