摘要
目的:基于微小RNA-9(miR-9)/核因子-κB(NF-κB)信号通路探讨丙泊酚对鼻咽癌细胞增殖、凋亡的影响。方法:设鼻咽癌细胞(CNE-2Z)采用细胞组、顺铂组(7.5μg·ml^(-1))、丙泊酚低、高剂量组(25,50μg·ml^(-1)),以上各组每孔设6个平行样,培养72 h,培养结束后,采用四唑盐(MTT)法及Giemsa溶液染色测定细胞增殖及克隆形成数目,Transwell腔室系统测定侵袭迁移水平,流式细胞仪测定凋亡水平,实时荧光定量PCR(qRT-PCR)法及蛋白免疫印迹(WB)法测定miR-9、NF-κB mRNA和蛋白水平。结果:与CNE-2Z细胞组比较,顺铂组、丙泊酚低、高剂量组吸光度(A)值、存活率、克隆形成数目、穿膜数、miR-9、NF-κB mRNA和蛋白水平显著降低,凋亡率显著升高(P<0.05);与顺铂组比较,丙泊酚低、高剂量组A值、存活率、克隆形成数目、穿膜数、miR-9,NF-κB mRNA和蛋白水平显著升高,凋亡率显著降低(P<0.05);丙泊酚剂量组间差异有统计学意义(P<0.05)。结论:丙泊酚可抑制鼻咽癌细胞增殖,促进其凋亡;其机制与丙泊酚抑制miR-9、NF-κB mRNA和蛋白表达进而抑制miR-9/NF-κB信号通路激活有关。
Objective: To explore the effects of propofol on the proliferation and apoptosis of nasopharyngeal carcinoma cells based on microRNA-9(miR-9)/nuclear factor-κB(NF-κB) signaling pathway.Methods: Nasopharyngeal carcinoma cells(CNE-2Z) cell group,cisplatin group(7.5 μg·ml^(-1)),propofol low-and high-dose groups(25 and 50 μg·ml^(-1)) were established,and each group had 6 parallel samples cultured for 72 h.After the culture,tetrazolium salt(MTT) method and Giemsa solution staining were used to determine the number of cell proliferation and clone formation,Transwell chamber system was used to measure the level of invasion and migration,flow cytometry was used to determine the level of apoptosis,and real-time quantitative PCR(qRT-PCR) and Western blotting(WB) were used to determine the levels of miR-9,NF-κB mRNA and protein.Results: Compared with those in CNE-2 Z cell group,the A value,survival rate,number of clone formation,number of transmembrane,and miR-9,NF-κB mRNA and protein levels were decreased significantly,while the rate of apoptosis increased significantly in cisplatin group and propofol low-and high-dose groups(P<0.05).Compared with those in cisplatin group,the A value,survival rate,number of clone formation,number of transmembrane,and miR-9,NF-κB mRNA and protein levels increased significantly,and the apoptosis rate decreased significantly in propofol low-and high-dose groups(P<0.05).The differences between the two propofol groups were also statistically significant(P<0.05).Conclusion: Propofol can inhibit the proliferation and promote the apoptosis of nasopharyngeal carcinoma cells;its mechanism is related to the inhibition of miR-9,NF-κB mRNA and protein expressions by propofol,and then inhibiting the activation of miR-9/NF-κB signaling pathway.
作者
许頔莎
蔡春妹
宋颖
Xu Disha;Cai Chunmei;Song Ying(Department of Anesthesiology,Characteristic Medical Center of Chinese People’s Armed Police Force,Tianjin 300161,China;Department of Anesthesiology,Shuige Hospital of Tianjin City)
出处
《中国药师》
CAS
2021年第3期467-471,481,共6页
China Pharmacist
