摘要
目的观察中药益气养阴消瘿方对自身免疫性甲状腺炎(AIT)模型大鼠B淋巴细胞活化的调节效应。方法4~5周雌性SD大鼠50只,按随机数字表法将其分为正常对照组,模型组,益气养阴消瘿方低、中、高剂量组,每组10只。以皮下注射猪甲状腺球蛋白并配合高碘水喂养制造AIT模型,益气养阴消瘿方低、中、高剂量组分别以益气养阴消瘿方溶液6、12、24 g/kg灌胃,正常对照组与模型组予以等量生理盐水灌胃,干预6周。酶联免疫吸附试验法检测各组大鼠血清促甲状腺激素(TSH)、甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)水平及B细胞活化因子(BAFF)的浓度;免疫组化法检测大鼠甲状腺组织白细胞介素-10(IL-10)的表达水平。结果与正常对照组比较,模型组血清TSH、TGAb、TPOAb水平及BAFF水平均升高,而甲状腺组织IL-10表达水平降低,差异均有统计学意义(均P<0.05)。与模型组比较,益气养阴消瘿方中、高剂量组TSH、TGAb、TPOAb浓度均降低,差异均有高度统计学意义(均P<0.01);益气养阴消瘿方低剂量组TGAb、TPOAb浓度均降低,差异有高度统计学意义(P<0.01);益气养阴消瘿方各剂量组BAFF含量均降低,甲状腺组织IL-10相对表达水平均升高,差异均有统计学意义(均P<0.05)。益气养阴消瘿方中、高剂量组TSH浓度均低于益气养阴消瘿方低剂量组,差异均有统计学意义(P<0.05或P<0.01);益气养阴消瘿方高剂量组TGAb浓度低于益气养阴消瘿方中、低剂量组,且益气养阴消瘿方中剂量组低于益气养阴消瘿方低剂量组,差异均有统计学意义(P<0.05或P<0.01)。益气养阴消瘿方高剂量组BAFF含量低于益气养阴消瘿方低剂、中剂量组,差异均有统计学意义(均P<0.05)。益气养阴消瘿方中、高剂量组IL-10表达水平均高于益气养阴消瘿方低剂量组,差异均有统计学意义(均P<0.05)。结论益气养阴消瘿方可降低AIT大鼠TSH、TGAb、TPOAb水平,改善免疫失衡状态,可能与其抑制B淋巴细胞活化及上调甲状腺IL-10表达的机制相关。
Objective To observe the regulating effect of Yiqi Yangyin Xiaoying Decoction on B lymphocyte activation in rats with autoimmune thyroiditis(AIT)model.Methods According to random number table method,50 female SD rats at four-five weeks were divided into normal control group,model group,low,medium and high dose Yiqi Yangyin Xiaoying Decoction groups,with 10 rats in each group.The AIT model was made by subcutaneous injection of porcine thyroglobulin and feed with high iodine water.The low,medium and high dose Yiqi Yangyin Xiaoying Decoction groups were given 6,12 and 24 g/kg solution of Yiqi Yangyin Xiaoying Decoction by gavage,respectively.Normal control group and model group were given the same amount of normal saline by gavage,intervention for six weeks.Serum levels of thyroid stimulating hormone(TSH),thyroid globulin antibody(TGAb),thyroid peroxidase antibody(TPOAb)and the concentration of serum B cell activating factor(BAFF)were determined by enzymy-linked immunosorbent assay.The expression level of interleukin-10(IL-10)in thyroid tissues of rats was detected by immunohistochemistry.Results Compared with the normal control group,serum TSH,TGAb,TPOAb levels and BAFF level in model group were increased,while the expression level of IL-10 in thyroid tissue was decreased,and the differences were highly statistically significant(all P<0.05).Compared with model group,the concentrations of TSH,TgAb and TPOAb in the medium and high dose Yiqi Yangyin Xiaoying Decoction groups were decreased,and the differences were highly statistically significant(all P<0.01);the concentration of TGAb and TPOAb in low dose Yiqi Yangyin Xiaoying Decoction group were decreased,and the differences were highly statistically significant(P<0.01);BAFF content in all dose groups of Yiqi Yangyin Xiaoying Decoction decreased,and the relative expression level of IL-10 in thyroid tissue increased,and the differences were statistically significant(all P<0.05).TSH concentrations in the medium and high dose Yiqi Yangyin Xiaoying Decoction groups were lower than those in the low dose Yiqi Yangyin Xiaoying Decoction group,and the differences were highly statistically significant(P<0.05 or P<0.01).The concentration of TGAb in the high dose Yiqi Yangyin Xiaoying Decoction group was lower than that in the medium and low dose Yiqi Yangyin Xiaoying Decoction groups,and that in the medium dose Yiqi Yangyin Xiaoying Decoction group was lower than that in the low dose Yiqi Yangyin Xiaoying Decoction group,and the differences were highly statistically significant(P<0.05 or P<0.01).The content of BAFF in the high dose Yiqi Yangyin Xiaoying Decoction group was lower than that in the low and medium dose Yiqi Yangyin Xiaoying Decoction groups,and the differences were statistically significant(all P<0.05).The expression level of IL-10 in the medium and high dose Yiqi Yangyin Xiaoying Decoction groups was higher than that in the low dose Yiqi Yangyin Xiaoying Decoction group,and the differences were statistically significant(all P<0.05).Conclusion Yiqi Yangyin Xiaoying Decoction decreases TSH,TGAb and TPOAb levels in AIT rats,and improves immune imbalance,which may be related to the mechanism of inhibiting B lymphocyte activation and upregulating thyroid IL-10 expression.
作者
李扬
牧亚峰
左新河
田曼
赵勇
LI Yang;MU Yafeng;ZUO Xinhe;TIAN Man;ZHAO Yong(Thyroid Disease Clinic Center,Hubei Provincial Hospital of Traditional Chinese Medicine,Hubei Province,Wuhan430070,China;Thyroid Disease Clinic Center,Hubei Province Academy of Traditional Chinese Medicine,Hubei Province,Wuhan430070,China;the First Clinical College,Hubei University of Chinese Medicine,Hubei Province,Wuhan430061,China)
出处
《中国医药导报》
CAS
2021年第8期15-19,33,共6页
China Medical Herald
基金
全国名老中医传承工作室建设项目(国中医药办人教函〔2018〕119号)
湖北省自然科学基金面上青年项目(2018CFB 278)。
