摘要
采用高通量扩增子测序法对秦巴山区镇巴、宁强和城固三地典型茶园茶树根际细菌多样性和茶树根际微环境进行分析。茶树根际细菌多样性研究结果表明,采样地样品中所有土壤茶树根际细菌分布在41个门(其中13个门相对丰度大于1%)、1288个属(其中33个属相对丰度大于1%)。镇巴采样地2年生茶树根际细菌数量最少,多样性最差。茶树根际微环境研究结果表明,所有采样地土壤的营养成分均能满足茶树生长需求,但城固采样地需要进行少量有机碳的补充;三个采样地土壤pH值在4.5~5.5,含水量呈现出不同变化趋势。茶树根际细菌多样性和根际微环境相关性分析结果表明,影响茶树根际细菌多样性的主要环境因子分别为土壤有机碳、有机氮及水解性氮含量。
Rhizosphere bacteria diversity and rhizosphere microenvironment of tea plants in Zhenba,Ningqiang,and Chenggu in Qinba Mountain area were analyzed by high throughput amplino sequencing method.The results of bacteria diversity in the rhizosphere of tea plant showed that,the bacteria in the samples were distributed in 41 phyla(13 phyla with relative abundance ratio greater than 1%)and 1288 genera(33 genera with relative abundance ratio greater than 1%)were found.The number and diversity of rhizosphere bacteria of two years old tea plants in the sampling area of Zhenba were the least and the worst.The results of rhizosphere microenvironment of tea plants showed that the nutrients in the soil of all sampling sites could meet the growth demand of tea plants,but a small amount of organic carbon supplement was needed in the Chenggu solid sampling sites;The soil pH values in the three sampling sites ranged from 4.5 to 5.5,and soil water content showed different trends.The results of correlation analysis between rhizosphere bacterial diversity and rhizosphere microenvironment showed that the main environmental factors affecting rhizosphere bacterial diversity were soil organic carbon,organic nitrogen and hydrolytic nitrogen.
作者
贺屹成
王梦姣
秦公伟
HE Yicheng;WANG Mengjiao;QIN Gongwei(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China)
出处
《茶叶通讯》
2021年第1期40-49,共10页
Journal of Tea Communication
基金
陕西省教育厅专项(19JK0191)
陕西省高校科协人才托举计划项目(20200206)
国家自然科学基金(31870488、41571300)
陕西省大学生创新创业训练项目(S201910720056)
陕西省高层次人才特殊支持计划(2017)。
关键词
茶树
秦巴山区
根际微生物
根际土壤元素含量
相关性
Tea plant
Qinba mountain area
Rhizospheric microorganism
Rhizosphere soil element content
Correlation
