摘要
食品中最常见的产毒真菌主要包括黄曲霉、赭曲霉、青霉属和镰刀菌属等,能够对人和动物机体造成不可逆转的伤害甚至死亡。针对产毒真菌检测可在毒素形成早期预测其产毒性能,为前置化干预毒素危害提供技术支撑,概述了近年来应用于食品中产毒真菌检测的常规PCR方法、实时荧光定量PCR方法和DNA指纹图谱方法、DNA条形码方法等,并对未来产毒真菌检测方法的发展趋势进行了展望。
The most common toxin-producing fungi in foods include Aspergillus flavus, Aspergillus ochraceus, Penicillium spp. and Fusarium spp., which can cause irreversible damage or even death to human and animal organisms. Detection of toxin-producing fungi can predict its toxin-producing performance in the early stages of toxin formation, and provide technical support for pre-treatment intervention of toxin damage. This paper summarized the conventional PCR methods, real-time fluorescent quantitative PCR method, DNA fingerprinting method and DNA barcode method for the detection of toxin-producing fungi in food, and prospected the development trend of toxin-producing fungi detection methods in the future.
作者
张庆
赵晓美
王娉
刘冰
陈颖
ZHANG Qing;ZHAO Xiaomei;WANG Ping;LIU Bing;CHEN Ying(State Key Laboratory of Food Nutrition and Safety,Key Laboratory of Food Nutrition and Safety,Ministry of Education,College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;Agro-Product Safety Research Center,Chinese Academy of Inspection and Quarantine,Beijing 100176,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2021年第3期91-98,共8页
Journal of Agricultural Science and Technology
基金
国家重点研发计划项目(2017YFC1601205)。
关键词
产毒真菌
核酸
检测方法
toxin-producing fungi
nucleic acid
detection method
