摘要
以183份甜、糯玉米自交系构成的关联群体为材料,在2种Cd污染水平下对甜、糯玉米开花期(DA,days to anthesis)、吐丝期(DS,days to silking)和开花吐丝间隔(ASI,anthesis silking interval)进行全基因组关联分析,以揭示重金属Cd胁迫对甜、糯玉米开花期的影响及调控基因。结果表明:不同Cd污染水平对甜、糯玉米DA和DS均产生影响,其中对DS的延长更为明显,从而导致ASI的增大。同时,甜玉米ASI平均值高于糯玉米ASI,由此表明甜玉米ASI对重金属Cd胁迫更为敏感。结合群体基因型和开花期相关性状表型的全基因组关联分析,分别筛选到3个开花期相关的SNP位点和6个吐丝期相关的SNP位点。根据其所对应的物理位置与Maize GDB和NCBI基因数据库进行比对,预测获得8个已知功能基因,其中包括在前人研究中与玉米开花期有关的候选基因。以上研究解析甜、糯玉米在重金属Cd污染水平下开花期及吐丝期的遗传规律,为今后开展甜、糯玉米分子标记辅助育种和Cd安全品种的选育提供参考。
In this study,183 sweet and waxy maize inbred lines were subjected to analyze the flowering time(DA,days to anthesis),silking time(DS,days to silking)and Anthesis silking interval(ASI)under Cdpolluted environments,in order to reveal the response and possible regulatory genes at the flowering stage.The results showed that Cd pollution prolonged DA and DS of sweet and waxy maize,especially for DS,which led to the increasing of ASI.The ASI value of waxy maize was found to be lower than that of sweet maize,implying the sensitivity of sweet corn under Cd stress.Three and six SNPs,which were associated with flowering time and silking time,respectively,were detected by Genome-wide association analysis(GWAS).These SNPs were found to be associated with eight genes annotated in maize GDB and NCBI gene databases,including few genes which were reported roles in the flowering time.Collectively,these results might provide theoretical reference for molecular marker assisted breeding and Cd safe breeding of sweet and waxy maize varieties in the future.
作者
袁亮
孟鑫
汪亚龙
廖长见
李高科
吕桂华
宋军
邱正高
林海建
YUAN Liang;MENG Xin;WANG Ya-long;LIAO Chang-jian;LI Gao-ke;LYU Gui-hua;SONG Jun;QIU Zheng-gao;LIN Hai-jian(Maize Research Institute of Chongqing Academy of Agricultural Science,Chongqing.401329;Maize Research Institule of Sichuan Agricultural University,Chengdu 611130;Crop Research Institute,Frujian Academy of Agricutural Sciences/Fujian Engineering Research Center for Characteristic Dry Crop Varieties Breeding,Fuzhou 350013;Crop research Instiute,Guangdong Academy of Agricultural Science,Guangzhou 510640;Institute of Maize and Featured Upland Crops,Zhejiang Academy of Agricultural Science,Dongyang 322105;Biotechnology and Nuclear Technology Research Institute of Sichuan Academy of Agricutural Sciences,Chengdu 610061)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2021年第2期438-447,共10页
Journal of Plant Genetic Resources
基金
重庆市技术创新与应用示范项目(cstc2018jscx-msyb X0241)
福建省属公益类科研院所专项(2018R1026-7,2019R1031-13)
福建省农业科学院科技创新团队项目(STIT2017-2-6)
重庆市农业科学院农业发展资金项目(NKY-2019AB014)。
关键词
甜、糯玉米
CD胁迫
开花期
关联分析
sweet and waxy maize
Cd stress
days to anthesis
correlation analysis
