摘要
目的:研究阿托伐他汀预处理对心肌缺血再灌注损伤大鼠心室重构、炎症反应和氧化应激的影响。方法:选取90只SD级大鼠进行研究,将其随机分成假手术组、缺血再灌注组、阿托伐他汀组,每组30只。假手术组与缺血再灌注组大鼠予以生理盐水(5 m L/d)连续灌胃7d处理,阿托伐他汀组予以阿托伐他汀20 mg/(kg·d)连续灌胃7 d,上述干预结束后,缺血再灌注组与阿托伐他汀组大鼠通过阻断大鼠冠状动脉左前降支的方式建立心肌缺血再灌注损伤模型。比较三组大鼠心室重构指标水平、炎症反应以及氧化应激相关指标水平。结果:缺血再灌注组、阿托伐他汀组大鼠的左室相对重量、右室相对重量、室间隔厚度、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、丙二醛(MDA)、乳酸脱氧酶(LDH)水平均高于假手术组,且阿托伐他汀组大鼠上述指标均低于缺血再灌注组(均P<0.05);缺血再灌注组、阿托伐他汀组大鼠白介素-10(IL-10)、超氧化物气化酶(SOD)水平低于假手术组,且阿托伐他汀组大鼠IL-10、SOD水平高于缺血再灌注组(均P<0.05)。结论:阿托伐他汀预处理可有效预防心肌缺血再灌注损伤大鼠心室重构,同时可在一定程度上改善大鼠的炎症反应和氧化应激反应。
Objective:To study the effects of atorvastatin preconditioning on ventricular remodeling,inflammatory response and oxidative stress in rats with myocardial ischemia-reperfusion injury.Methods:90 SD rats were selected for study,they were randomly divided into sham operation group,ischemia-reperfusion group and atorvastatin group,30 in each group.The rats in the sham operation group and the ischemia-reperfusion group were treated with normal saline(5 m L/d)continuous gavage for 7 days.Atorvastatin group was given atorvastatin at 20 mg/(kg·d)continuous gavage for 7 days.After the above intervention,the ischemia-reperfusion group and atorvastatin group established the myocardial ischemia-reperfusion injury model by blocking the left anterior descending branch of the coronary artery.The indexes of ventricular remodeling,inflammatory response and oxidative stress in the three groups were compared.Results:The relative weight of left ventricle,relative weight of right ventricle,ventricular septal thickness,the levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β),malondialdehyde(MDA),lactate deoxidase(LDH)of rats in the ischemia-reperfusion group and atorvastatin group were higher than those in the sham operation group,and all above indexes of rats in the atorvastatin group were lower than the ischemia-reperfusion group(all P<0.05).The levels of interleukin-10(IL-10)and superoxide gasification enzyme(SOD)of rats in the ischemia-reperfusion group and atorvastatin group were lower than those in the sham operation group,and the levels of IL-10,SOD in the atorvastatin group were higher than the ischemia-reperfusion group(all P<0.05).Conclusion:Atorvastatin preconditioning can effectively prevent ventricular remodeling in rats with myocardial ischemia-reperfusion injury,and improve the inflammatory response and oxidative stress in rats to a certain extent.
作者
赵灿
王永亮
沈絮华
彭晖
吴永全
ZHAO Can;WANG Yong-liang;SHEN Xu-hua;PENG Hui;WU Yong-quan(Department of Cardiology,Beijing Friendship Hospital,Capital Medical University,Beijing,100050,China;Department of Cardiology,Beijing Anzhen Hospital,Capital Medical University,Beijing,100029,China)
出处
《现代生物医学进展》
CAS
2021年第4期614-617,共4页
Progress in Modern Biomedicine
基金
北京市自然科学基金项目(7145214)。
关键词
心肌缺血再灌注损伤
阿托伐他汀
心室重构
炎症反应
氧化应激
Myocardial ischemia reperfusion injury
Atorvastatin
Ventricular remodeling
Inflammatory response
Oxidative stress
