摘要
目的检测桥本甲状腺炎(hashimoto thyroiditis, HT)患者和健康体检者血浆中B细胞活化因子(B-cell activating factor, BAFF)和白介素21(IL-21)表达水平,探讨二者在HT发病机制中的作用。方法选取2019年12月—2020年6月蚌埠医学院第一附属医院内分泌门诊就诊HT患者82例作为观察组(HT组),并根据甲状腺功能将HT组分成NHT组(甲状腺功能正常,39例)和SHT组(甲状腺功能减退或亚临床甲减,43例),选取同期健康体检者51例作为对照组(NC组)。收集受试者外周静脉血血浆,酶联免疫吸附实验(ELISA)检测研究对象血浆BAFF、IL-21,化学发光免疫法检测甲状腺功能及相关抗体等指标。使用SPSS 24.0统计学软件对数据进行详细分析。结果 HT组患者血浆中BAFF、IL-21表达水平较NC组明显上调(均P<0.05)。HT患者血浆中BAFF与甲状腺球蛋白抗体(TgAb)、甲状腺微粒体抗体(TMAb)水平呈正相关(r=0.444、0.401,均P<0.05),IL-21与TgAb、TMAb水平呈正相关(r=0.451、0.451,均P<0.05),BAFF与IL-21呈正相关(r=0.324,P<0.05),BAFF与总甲状腺素(TT4)呈负相关(r=-0.214,P<0.05)。BAFF和IL-21(AUC=0.896)联合诊断HT较BAFF(AUC=0.862)、IL-21(AUC=0.809)单独诊断价值更高。结论 BAFF与IL-21水平与HT病变有关,与TgAb、TMAb水平密切相关,可能参与HT的发生发展过程。
Objective To investigate the expression of human B cell activating factor(BAFF)and interleukin-21(IL-21)in the plasma of patients with Hashimoto’s thyroiditis(HT)and explore their roles in the pathogenesis of HT.Methods Patients with HT in the Endocrinology Clinic of The First Affiliated Hospital of Bengbu Medical College from December 2019 to June 2020 were selected as the observation group(HT group,n=82).According to thyroid functions,the HT group was further divided into an NHT group(normal thyroid function,n=39)and an SHT group(hypothyroidism or subclinical hypothyroidism,n=43).A total of 51 patients(NC group)who visited the hospital for consultation during the same period were designated as the control group.Peripheral venous blood plasma was collected.BAFF and IL-21 were tested via enzyme-linked immunosorbent assay(ELISA).Thyroid function,thyroid microsomal antibody(TMAB)and thyroglobulin antibody(TGAB)were detected via chemiluminescence immunoassay.Data were analysed using SPSS 24.0.Results The levels of BAFF and IL-21 in the plasma of the HT group were significantly higher than those of the NC group(all P<0.05).The levels of BAFF in the SHT group was significantly higher than that in the NHT group(P<0.05).The plasma levels of BAFF in the patients with HT were positively correlated with TGAB and TMAB(r=0.444 and 0.401,respectively;all P<0.05).Similarly,IL-21 was positively correlated with TGAB and TMAB(r=0.451 and 0.451,respectively;all P<0.05).A positive correlation was observed between BAFF and IL-21(r=0.324,P<0.05).By contrast,a negative correlation was found between BAFF and total T4(TT4,r=-0.214,P<0.05).The combined diagnosis of HT with BAFF and IL-21(AUC=0.896)was more valuable than that of BAFF(AUC=0.862)and IL-21(AUC=0.809)alone.Conclusion BAFF combined with IL-21 has a certain value in the diagnosis of HT.BAFF and IL-21 play an important role in the occurrence and development of HT.Moreover,they participate in the maturation,proliferation and differentiation of B cells.Finally,they participate jointly in the production of TGAB and TMAR.
作者
何程程
金国玺
毕娅欣
HE Cheng-cheng;JIN Guo-xi;BI Ya-xin(Department of Endocrinology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)
出处
《中华全科医学》
2021年第4期568-571,共4页
Chinese Journal of General Practice
基金
安徽省教育厅重大项目(KJ2019ZD29)。
