摘要
目的探究芬戈莫德(FTY720)对局灶性脑缺血再灌注大鼠模型炎症)的影响。方法8周龄(250 g~300 g)SD大鼠雌雄各半,分为假手术组、空白对照组、氯吡格雷组、FTY720组。应用线栓法制作大鼠缺血性卒中模型,不同组别经不同药物处理后进行行为障碍评分,并应用TTC染色观察脑缺血面积及HE染色观察缺血组织神经元损伤情况,取各组大鼠海马组织应用ELISA法测定炎症因子IL-1β及IL-6水平,Western-blot法测定p-p38MAPK及NF-κB水平。结果(1)FTY720可减轻模型大鼠行为障碍(P<0.01);(2)FTY720可缩小模型大鼠脑组织梗死面积(P<0.01)及减轻神经元损伤;(3)FTY720可降低大鼠缺血区脑组织中IL-1β(P<0.01)、IL-6(P<0.01)及p-p38MAPK(P<0.01)、NF-κB(P<0.01)水平。结论FTY720对局灶性脑缺血再灌注大鼠模型脑组织具有保护作用,其机制可能通过p38MAPK通路进而影响炎症反应。
Objective To investigate the effect of Fingomod(FTY720)on the cerebral ischemia-reperfusion injury in rats.Methods SPF rats(250 g~300 g)of 8 weeks old were divided into sham operation group,blank control group,group of Clopidogrel and group of FTY720.Rat model of ischemic stroke was made by tMCAO.The behavioral disorder scores were scored in different groups after different drug treatments.And observing the area of infarctus tissue by the TTC method and the nerve cells changed by HE method in different groups.The levels of inflammatory factors IL-1βand IL-6 in brain tissue of rats were changed by method of ELISA.The level of p-p38MAPK and NF-κB in brain tissue of rats were determined by Western-blot method.Results(1)FTY720 can alleviate the behavior disorder of rats(P<0.01);(2)FTY720 can reduce the area of infarctus tissue(P<0.01)and the injury of neurology cells in rats;(3)FTY720 can reduce the level of p-p38MAPK and NF-κB(P<0.01)in the ischemic brain tissue of rats;4,FTY720 can be reduced The level of IL-1β(P<0.01)and IL-6(P<0.01)in the ischemic brain tissue.Conclusion Fingomod(FTY720)has a protective effect on the cerebral ischemia-reperfusion rat model by decreasing the level of inflammatory response.
作者
李娜
张磊
王建楠
李静
乔嘉璐
王奕丹
隋汝波
LI Na;ZHANG Lei;WANG Jiannan(Department of Neurology,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China)
出处
《中风与神经疾病杂志》
CAS
2021年第3期196-200,共5页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金(No.81371461)
辽宁省教育厅科技项目(No.JYTJCZR201903)
辽宁省教育厅青年基金项目(No.JYTQN201707)。
