摘要
以L-色氨酸为印迹分子,S-2-巯基丙酸为手性功能单体,分别制备了25个L-色氨酸分子印迹复合膜(MICM_(1~25))及相应的非印迹复合膜(NICM_(1~25))。采用扫描电镜(SEM)表征最优印迹复合膜(MICM_(5))及相应的非印迹复合膜(NICM_(5))的外观形态,通过等温吸附模型对印迹复合膜的吸附性能进行评价,并结合高效液相色谱法(HPLC法)分析MICM_(5)对外消旋体DL-色氨酸的手性拆分能力。结果表明:以聚四氟乙烯膜为支撑膜,印迹分子、功能单体和交联剂的摩尔比为1∶8∶90,甲醇∶水(1∶1,体积比)为致孔剂可制备出较优的分子印迹复合膜,其对外消旋体DL-色氨酸有较好的手性拆分能力,拆分因子达到2.41。相对于常用功能单体制备的L-色氨酸分子印迹复合膜,采用S-2-巯基丙酸为手性功能单体制备的L-色氨酸分子印迹复合膜具有更高的亲和性和更好的手性拆分能力。
Using L-tryptophan as imprinted molecule and S-2-mercaptopropionic acid as chiral functional monomer,25 L-tryptophan molecular imprinted composite membranes(MICM_(1~25))and corresponding non-imprinted composite membranes(NICM_(1~25))were prepared,respectively.The appearance of the optimal composite membrane(MICM_(5))and the corresponding non-imprinted composite membrane(NICM_(5))were characterized by scanning electron microscope(SEM),and the adsorption performance of the membrane was evaluated by the isothermal adsorption model.The chiral resolution ability of the racemic DL-tryptophan was also investigated by high performance liquid chromatography.The results showed that the optimal experimental conditions were as follows:the polytetrafluoroethylene membrane was used as the base membrane,the molar ratio of imprinting molecule,functional monomer and crosslinking agent was 1∶8∶90,the porogen was methanol∶water(v∶v,1∶1),optimal molecularly imprinted composite membrane was prepared.It showed a desired chiral resolvability for the racemic DL-tryptophan with a resolution factor of 2.41.The L-tryptophan molecular imprinted composite membrane prepared with S-2-mercaptopropionic acid as functional monomer had higher affinity and better chiral resolution ability than the prepared by common functional monomers.
作者
刘迎梅
郝维成
赵莉
胡德琼
字富庭
胡显智
成会玲
Liu Yingmei;Hao Weicheng;Zhao Li;Hu Deqiong;Zi Futing;Hu Xianzhi(Cheng Huiling,Faculty of Science,Kunming University of Science and Technology,Kunming 650500)
出处
《化工新型材料》
CAS
CSCD
北大核心
2021年第3期90-94,共5页
New Chemical Materials
基金
国家自然科学基金(地区科学基金)(21764008)。
关键词
L-色氨酸
S-2-巯基丙酸
分子印迹复合膜
手性拆分
L-tryptophan
S-2-mercaptopropionic acid
molecularly imprinted composite membrane
chiral resolution
