摘要
目的:探讨不同浓度聚乙二醇-b-聚L-苯丙氨酸嵌段聚合物凝胶(PEBP凝胶)对大鼠子宫内膜损伤导致的纤维化的抑制作用。方法:24只雌性SD大鼠随机分为4组(B、C、D、E组,n=6),采用刮宫兼感染双重损伤法制作大鼠宫腔纤维化模型。B、C、D、E组双侧子宫造模后,随机一侧子宫注入PEBP浓度分别为0、8.125、16.25、32.5 mg/mL的凝胶0.3 mL;另一侧子宫注入0.3 mL生理盐水作为模型组(A组)。损伤14 d后取子宫组织固定、包埋、切片,行苏木素伊红(HE)染色以观察各组大鼠的子宫愈合情况,行Masson染色以观察子宫内膜纤维化程度,并通过免疫组化法检测子宫内膜组织中转化生长因子β1(TGF-β1)的表达情况。结果:实验组与模型组相比,损伤后宫内植入PEBP凝胶可显著缓解损伤造成的子宫内膜纤维化(P<0.05),缓解程度随PEBP在凝胶中浓度的升高而升高;PEBP凝胶组子宫内膜组织TGF-β1的表达随凝胶中PEBP含量的增加而降低。与模型组相比,PEBP含量最高的E组TGF-β1的表达显著降低(P<0.05)。结论:PEBP凝胶对大鼠子宫内膜损伤导致的纤维化具有抑制作用,且抑制作用随着凝胶中PEBP浓度的升高而增强,高浓度PEBP凝胶也可抑制损伤后子宫内膜TGF-β1的表达。
Objective:To evaluate the effect of different dose of poly(ethyleneglycol)-b-poly(L-phenylalanine)block polymer(PEBP)gel on inhibition of fibrosis induced by endometrial injury in rats.Methods:A total of 24 female SD rats were randomly divided into groups B,C,D and E(n=6).The model of intrauterine adhesions was established by double injury of curettage and infection in rats.For rats in each group,one side of their uterus was given 0.3 mL of PEBP gel with a dose of 0,8.125,16.25 and 32.5 mg/mL respectively after modeling,while the other side of their uterus was treated with 0.3 mL saline,serving as the model group(group A).14 d after injury,the uterine tissues were fixed,embedded and sliced.Hematoxylin and eosin(HE)and Masson staining was respectively used to observe the healing of uterus and the degree of endometrial fibrosis.Also,the expression of transforming growth factor-β1(TGF-β1)in endometrium was detected by immunohistochemistry.Results:Compared with the model group,intrauterine implantation of PEBP gel significantly alleviated the endometrial fibrosis induced by injury(P<0.05),and the degree of remission increased with the increase of PEBP concentration in the gel.The expression of TGF-β1 in endometrial tissue of PEBP gel group decreased with the increase of PEBP content in the gel.The expression of TGF-β1 was significantly decreased in E group with the highest PEBP gel concentration than the model group(P<0.05).Conclusion:PEBP gel inhibited the fibrosis induced by endometrial injury in rats,and the inhibitory effect increased with the increase of PEBP dose in the gel.The high-dose PEBP gel also inhibited the expression of TGF-β1 in the endometrium after injury.
作者
党家凤
谌伦华
张娟
沈成义
王冰
DANG Jia-feng;SHEN Lun-hua;ZHANG Juan;SHEN Cheng-yi;WANG Bing(School of Preclinical Medicine,School of Preclinical Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Department of Gynecology and Obstetrics of Affiliated Hospital,School of Preclinical Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Morphological Research,School of Preclinical Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Sichuan Key Laboratory of Medical Imaging,School of Preclinical Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Department of Chemistry,School of Preclinical Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)
出处
《川北医学院学报》
CAS
2021年第3期279-282,共4页
Journal of North Sichuan Medical College
基金
2020中央引导地方科技发展专项自由探索基础项目(2020ZYD056)。
