摘要
目的:研究血管内皮生长因子(VEGF)-A及磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)通路在结直肠癌中的表达及临床意义。方法:选取2013年9月-2015年9月本院收治的结直肠癌患者80例作为观察组,同时切取患者癌旁正常肠黏膜组织作为对照组。分别采用免疫组化法检测癌组织和癌旁组织VEGF-A、PI3K及AKT表达水平,统计比较两组三指标表达阳性率。单因素分析结直肠癌组织中VEGF-A、PI3K及AKT表达水平与患者临床病理资料的相关性,同时Spearman相关性分析VEGF-A与PI3K及AKT表达相关性。术后随访5年,比较VEGF-A、PI3K及AKT阳性和阴性表达患者生存率。结果:观察组VEGF-A、PI3K及AKT表达阳性率均明显高于对照组(P<0.05)。结直肠癌VEGF-A、PI3K及AKT阳性表达患者性别、年龄、肿瘤直径、肿瘤部位比较,差异均无统计学意义(P>0.05);结直肠癌VEGF-A、PI3K及AKT阳性表达患者TNM分期、分化程度、淋巴结转移及浸润深度比较,差异均有统计学意义(P<0.05)。结直肠癌组织中VEGF-A表达与PI3K、AKT表达均呈明显正相关(rs=0.625、0.517,P=0.001)。结直肠癌中VEGF-A、PI3K及AKT阳性表达患者5年生存率均明显低于阴性表达患者(P<0.05)。结论:结直肠癌组织中VEGF-A、PI3K及AKT均存在高表达现象,与肿瘤分期、淋巴结转移、分化及浸润深度呈明显相关性,且患者生存期更短,可能参与结直肠癌病情发生发展,对临床诊断及治疗具有重要意义。
Objective:To study the expression and clinical significance of vascular endothelial growth factor(VEGF)-A and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway in colorectal cancer.Method:From September 2013 to September 2015,80 patients with colorectal cancer were selected as the observation group,at the same time the normal intestinal mucosal tissue was cut off as the control group.The VEGF-A,PI3K and AKT expression levels of cancer tissues and adjacent tissues were detected by immunohistochemistry,the positive expression rate of three indexes in two groups were counted and compared.Univariate analysis of the correlation between VEGF-A,PI3K and AKT expression in colorectal cancer and clinicopathological data,the correlation of VEGF-A with PI3K and Akt expression was by Spearman correlation analysis.The patients were followed up for 5 years,the survival rate of VEGF-A,PI3K and AKT positive and negative expression patients were compared.Result:The positive rate of VEGF-A,PI3K and AKT expression in the observation group were significantly higher than those in the control group(P<0.05).There were no significant differences in sex,age,tumor diameter and tumor site of colorectal cancer VEGF-A,PI3K and AKT positive expression patients(P>0.05);there were significant differences in stage,differentiation degree,lymph node metastasis and invasion depth of colorectal cancer VEGF-A,PI3K and positive expression patients(P>0.05).There were significant positive correlation between VEGF-A expression and PI3K,AKT expression in colorectal cancer(rs=0.625,0.517,P=0.001).The survival rate of colorectal cancer VEGF-A and PI3K and AKT positive expression patients were significantly lower than those of negative expression patients(P<0.05).Conclusion:VEGF-A,PI3K and AKT in colorectal cancer tissues are highly expressed,and shows significant correlation with tumor stage,lymph node metastasis,differentiation and depth of invasion,it also reduces survival,may be involved in the development of colorectal cancer,it is of great significance for clinical diagnosis and treatment.
作者
徐润强
刘宇平
张海良
刘海盛
刘钰君
姚金科
XU Runqiang;LIU Yuping;ZHANG Hailiang;LIU Haisheng;LIU Yujun;YAO Jinke(Zengcheng District People’s Hospital of Guangzhou City,Guangzhou 511300,China;不详)
出处
《中国医学创新》
CAS
2021年第3期1-5,共5页
Medical Innovation of China
基金
广州市科技计划项目(201904010074)。
