摘要
以麦麸、玉米粉、稻壳、秸秆、甘蔗渣等固体基质为原料,采用单因素和正交试验,对棘孢木霉DQ−1分生孢子固体发酵基质和发酵条件进行了筛选和优化;并通过盆栽试验研究了其发酵产物对黄瓜、辣椒、番茄和西瓜幼苗生长的影响。结果表明:以单一麦麸为发酵基质时,棘孢木霉DQ−1产孢量最大,发酵5 d产孢量为1.51×109 CFU/g;以麦麸:玉米粉:稻壳:硅藻土复配比例为5.8:0.5:1.7:1.0时产孢量最大,发酵5 d棘孢木霉DQ−1产孢量可达2.63×10^(9) CFU/g。固体发酵条件以液体接种量为2%时,发酵第12 d棘孢木霉DQ−1的产孢量最高,可达7.18×10^(9) CFU/g。盆栽试验表明,棘孢木霉菌DQ−1分生孢子粉对黄瓜、辣椒、番茄和西瓜幼苗具有明显的促进生长效果。本研究结果为棘孢木霉菌DQ−1产业化生产和应用奠定了基础。
In this study,the solid fermentation substrate and conditions of Trichoderma asperellum DQ−1 were screened and optimized by single factor and orthogonal test using wheat bran,corn flour,rice husk,straw and bagasse as raw materials.The effects of fermentation products on the growth of cucumber,pepper,tomato and watermelon seedlings were investigated by pot experiment.Single factor tests showed that T.asperellum DQ−1 with single wheat bran as the fermentation substrate could produce the largest amount of conidia of,and the amount of conidia was 1.51×10^(9) CFU/g after 5 days of fermentation.When wheat bran,corn flour,rice husk and diatomaceous earth were combined at the proportion of 5.8:0.5:1.7:1,the conidia yield of T.asperellum DQ−1 reached 2.63×10^(9) CFU/g after 5 days of fermentation.Under the condition of solid fermentation with liquid inoculation at the inoculation amount of 2%,the conidia yield of T.asperellum DQ−1 was the highest,up to 7.18×10^(9) CFU/g,on the 12th day of fermentation.The pot experiment demonstrated that the T.asperellum DQ−1 conidia powder had obvious growth promotion effect on the seedlings of cucumber,pepper,tomato and watermelon.The above results will lay a foundation for the industrial production and application of T.asperellum DQ−1.
作者
张成
廖文敏
薛鸣
陈迪
侯巨梅
刘铜
ZHANG Cheng;LIAO Wenmin;XUE Ming;CHEN Di;HOU Jumei;LIU Tong(Engineering Center of Agricultural Microbial Preparation Research and Development of Hainan(Hainan University),Haikou 570228,China;Plant Protection College of Hainan University,Haikou 570228,China;Haikou R& D Center of Guizhou Yibaiyi Biotechnology Co.,Ltd,Haikou 570100,China)
出处
《中国生物防治学报》
CSCD
北大核心
2021年第2期315-322,共8页
Chinese Journal of Biological Control
基金
海南省重点研发项目(ZDYF2019064)
海南省高等学校科学研究项目(Hnky2019ZD-4)。
关键词
棘孢木霉
固体发酵
促进生长
Trichoderma asperellum
solid fermentation
growth promotion
