摘要
目的建立三七中20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3的含量测定方法,评价不同药用部位及加工方式的三七提取物中,两个型的人参皂苷Rg3的含量情况。方法采用高效液相色谱法测定,色谱条件:ECOSIL 120-5-C18-SH色谱柱(4.6×250mm,5μm);流动相为乙腈-异丙醇-水(47:3:50);流速:1.0ml·min^(-1);检测波长:203nm;进样量:10μL;柱温:20℃;测定不同药用部位及加工方式的三七提取物中20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3的含量。结果20(S)-人参皂苷Rg3的含量测定线性范围14.0~279.6μg·ml^(-1),(r2=1.0000),平均加样回收率100.56%,RSD2.46%。20(R)-人参皂苷Rg3的含量测定线性范围14.1~282.0μg·ml^(-1),(R^(2)=0.9999),平均加样回收率101.51%,RSD2.99%。结论该测定方法简便、稳定、高效;三七不同药用部位中根材优于其他药用部位;熟三七中20(S)-人参皂苷Rg3含量明显升高。
Objective To establish a method for the determination of 20(s)-Ginsenoside Rg3 and 20(R)-Ginsenoside Rg3 in Panax notoginseng,and to evaluate the content of two types of Ginsenoside Rg3 in Panax notoginseng extracts from different medicinal parts and processing methods.Methods HPLC was used to determine the content.The chromatographic conditions were as follows:ecosil 120-5-c18-sh column(4.6×250mm,5μm);The mobile phase was acetonitrile-isopropanol-water(47:3:50);the flow rate was 1.0ml·min^(-1);the detection wavelength was 203nm;the injection volume was 10μL;the column temperature was 20℃;the contents of 20(s)-Ginsenoside Rg3 and 20(R)-Ginsenoside Rg3 in different medicinal parts and processing methods of Panax notoginseng extract were determined.Results the linear range of 20(s)-Ginsenoside Rg3 was 14.0~279.6μg·ml^(-1)(r^(2)=1.0000).The average recovery was 100.56%,RSD 2.46%.The linear range of 20(R)-Ginsenoside Rg3 was 14.1~282.0μg·ml^(-1)(R^(2)=0.9999).The average recovery was 101.51%,RSD 2.99%.Conclusion The content of 20(s)-Ginsenoside Rg3 in cooked Panax notoginseng increased significantly.
作者
孙媛
田秀秀
丁江生
SUN Yuan;TIAN Xiu-xiu;DING Jiang-sheng(Yunnan pharmaceutical research institute,Kunming 650111,China;Innovation and R&D center of Yunnan Baiyao group,Kunming 650111,China;Key laboratory of new drug creation enterprises of traditional Chinese medicine and ethnic medicine in Yunnan province,Kunming 650111,China)
出处
《现代中医药》
CAS
2021年第2期29-34,共6页
Modern Chinese Medicine
基金
云南省科技厅“重大科技专项(生物医药)”(2018ZF013)。
