摘要
研究不同浓度6-BA、NAA组合对软枣猕猴桃(Actinidia arguta)带腋芽嫩枝的芽诱导、芽增殖与生根的影响以及软枣组培苗的移栽技术。结果表明,带腋芽嫩枝做外植体,经诱导,腋芽萌发获得无菌苗,最佳芽诱导培养基为MS+1.0 mg/L 6-BA+0.8 mg/L NAA,猕枣1号和猕枣2号的腋芽萌发率分别为95.8%和97.5%。获得的软枣无菌苗切成带2~3片叶的茎段,接种于芽增殖培养基上增殖培养,最佳芽增殖培养基为MS+1.0 mg/L 6-BA+0.25 mg/L NAA,芽周期增殖系数分别可达4.3和6.3。最佳生根培养基为1/2MS+0.5 mg/L IBA,生根率均达100%。生根组培苗直接移栽至智能温室,移栽3个月后成活率均可达95%以上。
The effects of different concentrations of 6-BA and NAA on bud induction,bud proliferation and rooting of stem section with axillary buds of Actinidia arguta were studied in this research.The stem section with axillary buds were choose as explants,which were induced to sterile seedlings with the best bud inducing medium MS+1.0 mg/L 6-BA+0.8 mg/L NAA.The germination rate from axillary buds of Actinidia arguta mizao No.1 and Actinidia arguta mizao No.2 were 95.8%and 97.5%respectively.Sterile seedlings of mizao No.1 and mizao No.2 were cut into stems with 2~3 leaves,which were inoculated on the bud proliferation medium(MS+1.0 mg/L 6-BA+0.25 mg/L NAA).The growth coefficient of proliferation buds of mizao No.1 and mizao No.2 during a 45-day cycle are 4.3 and 6.3,respectively.The best rooting medium was 1/2MS+0.5 mg/L IBA,which made 100%rooting rate.The survival rate of rooting tissue culture seedlings can reach more than 95%three months after transplanting.
作者
吕海燕
李大卫
费早霞
钟彩虹
LYU Hai-yan;LI Da-wei;FEI Zao-xia;ZHONG Cai-hong(Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture/Wuhan Botanical Garden/Engineering Laboratory for Kiwifruit Industrial Technology,Chinese Academy of Sciences,Wuhan 430074,China)
出处
《湖北农业科学》
2021年第7期116-119,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31572092,31701974)
湖北省科技创新创业服务能力建设专项(2018BEC475)
湖北省技术创新专项(重大项目)(2016ABA109)
中国科学院科技服务网络计划项目(KFJ-EW-STS-076)。
关键词
猕枣1号
猕枣2号
腋芽诱导
芽增殖
生根
Actinidia arguta mizao No.1
Actinidia arguta mizao No.2
axillary buds induction
bud proliferation
rooting
