摘要
目的:观察低频耳电针在降低内脏高敏感模型大鼠内脏敏感性中的作用。方法:SD大鼠随机分为空白组、模型组、耳针1组、耳针2组,每组10只。除空白组外,其余大鼠通过母婴分离加醋酸灌肠建立内脏高敏感大鼠模型。耳针1组与耳针2组接受2 Hz电针刺激30 min/d,10 d。通过腹外斜肌肌电(EMG)测定直肠扩张下大鼠内脏疼痛阈值,远端结肠HE染色观察病理改变,实时荧光定量PCR检测大鼠下丘脑nesfatin-1,促皮质素释放因子(CRF)及其受体1,2(CRF1/2R)及结肠5-羟色胺受体4(serotonin receptor 4,5-HT4R)的mRNA表达情况。结果:模型组腹外斜肌放电曲线下面积(AUC)较空白组显著增加(P<0.05);与模型组相比,耳针1组AUC显著减少(P<0.05)。与空白组比较,模型组下丘脑CRF、nesfatin-1、CRF1R、CRF2R的mRNA表达均显著升高(P<0.05),远端结肠5-HT4R的mRNA表达有下降的趋势(P=0.067)。耳针1组下丘脑CRF、nesfatin-1、CRF1R、CRF2R的m RNA较模型组显著下降(均P<0.05),且远端结肠5-HT4R的mRNA表达显著提高(P<0.05)。耳针2组下丘脑CRF、CRF2R的mRNA较模型组显著下降(均P<0.05),但较模型组nesfatin-1、CRF1R无显著差异,远端结肠5-HT4R的mRNA表达上调不显著(P>0.05)。结论:低频耳电针可缓解内脏高敏感大鼠的内脏敏感性,可能与调节下丘脑CRF、CRF1R、CRF2R、nesfatin-1,以及远端结肠5-HT4R的mRNA表达有关。
Objective:This study aims to explore the role of‘Brain-gut’regulation in low-frequency auricular electro-acupuncture on maternal separation combined with enema induced visceral hypersensitivity rats. Methods:Visceral hypersensitivity was established by maternal separation combined with acetic acid enema in SD rats,which were randomly divided into the normal control(NC)group,model control(MC)group,auricular acupuncture group 1(AA1)and auricular acupuncture group 2(AA2). Rats in the AA1 group and AA2 group were received electroacupuncture stimulation(2 Hz,30 min/d)for consecutive 10 days. The visceral sensitivity of rats was evaluated by electromyographic(EMG)to graded colorectal distension. The pathological change in distal colon were observed by HE staining. The mRNA expressions of hypothalamic corticotropin releasing factor(CRF),corticotropin releasing factor receptor1(CRF1R),corticotropin releasing factor receptor 2(CRF2R),nesfatin-1,and colonic serotonin receptor 4(5-HT4R)were detected by quantitative real-time PCR. Results:Rats in MC group show higher EMG activity,and mRNA expression of hypothalamic CRF,nesfatin-1,CRF1R,and CRF2R compared with NC group(all P < 0.05). The colonic 5-HT4R mRNA expression in MC group shows a decreased tendency(P=0.067). In contrast,AA treatment significantly reduced the area under the curve(AUC)(P <0.05). Further,compared to MC group,the mRNA expressions of hypothalamic CRF,nesfatin-1,CRF1R,and CRF2R of AA group significantly decreased(P<0.05)while the colonic 5-HT4R mRNA expression elevated(P<0.05). Conclusion:Low-frequency auricular electro-acupuncture could ameliorate the visceral sensitivity in rats with visceral hypersensitivity,which might relate to the regulation of mRNA expression of CRF,nesfatin-1,CRF1R,CRF2R in hypothalamus and of 5-HT4R in colon.
作者
徐万里
周帅
周静珠
赵瑞瑞
孙鲁宁
王永庆
薛明新
陈欢
张朝晖
XU Wanli;ZHOU Shuai;ZHOU Jingzhu;ZHAO Ruirui;SUN Luning;WANG Yongqing;XUE Mingxin;CHEN Huan;ZHANG Zhaohui(Department of Acupuncture,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029;the Second Clinical School of Medicine,Nanjing University of Traditional Chinese Medicine,Nanjing 210029;Department of Pharmacy,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第2期181-186,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省中医药科技项目(YB201789)
江苏省“六大人才高峰”高层次人才项目(2016-WSN-094)
江苏省第三批中医临床优秀人才研修项目。
