摘要
华重楼灰霉病在湖北和湖南两省多有发生,已对华重楼药材生产构成了严重威胁。为明确该病菌的遗传多样性水平,本研究对分离自两省10个地区的92株华重楼灰霉病菌进行了rDNA-ITS分子鉴定,同时采用SSR-PCR技术进行了遗传多样性分析。rDNA-ITS分子鉴定结果表明92株病原菌均为灰葡萄孢Botrytis cinerea,ITS系统发育树可初步显示其遗传多样性丰富。SSR-PCR多样性分析结果表明,应用9对SSR引物共检测出109个等位基因位点,等位基因和有效等位基因平均值分别为12和3.6589,香农指数和多态信息含量平均值分别为1.5048和0.6196。10个群体各自有效等位基因在1.622-3.971之间;香农指数在0.527-1.582之间;观测杂合度在0.148-0.593之间;期望杂合度在0.321-0.708之间。Nei遗传距离为0.15时,UPGMA聚类树可将92株病菌划分为4个类群。群体间基因流值为0.19-4.67,遗传分化值为0.047-0.638,群体遗传同一性和遗传距离范围值分别为0.1531-0.9679和0.0327-1.8766。综上所述,湖北和湖南两省华重楼灰霉病菌群体存在明显的遗传多样性,这为湖北和湖南两省区华重楼灰霉病的科学防治提供了理论依据。
Botrytis disease is the most common and serious disease on Paris polyphylla var.chinensis in Hubei and Hunan provinces.In order to clarify the genetic diversity level of the pathogen Botrytis cinerea,92 isolates from 10 regions of Hubei and Hunan provinces were analyzed by rDNA-ITS molecular identification and simple sequence repeats(SSR-PCR)molecular marker technique.rDNA-ITS molecular identification showed that these isolates were all B.cinerea and ITS phylogeny tree indicated significant genetic diversity.SSR-PCR diversity analysis showed that 109 alleles were detected by 9 primer pairs,and observed number of alleles and effective number of alleles average 12 and 3.6589 respectively.Shannon’s diversity index was1.5048,and polymorphism information content 0.6196.For the investigated 10 regions,the values of effective number of alleles ranged from 1.622 to 3.971,with Shannon’s diversity index of 0.527-1.582,observed heterozygosity of 0.148-0.593,and expected heterozygosity of0.321-0.708.The UPGMA phylogenetic tree based on Nei’s genetic distance showed that fungal isolates from 10 regions were divided into four groups.Gene flow value between populations was 0.19-4.67,with genetic differentiation value of 0.047-0.638,genetic identity value of0.1531-0.9679,and genetic distance value of 0.0327-1.8766.In summary,the populations of B.cinerea on Paris polyphylla var.chinensis in Hubei and Hunan provinces were genetically diverse,and the results may benefit the control of the disease in these provinces.
作者
王帆帆
唐涛
曾佳
郭杰
唐其
郭晓亮
段媛媛
游景茂
WANG Fan-Fan;TANG Tao;ZENG Jia;GUO Jie;TANG Qi;GUO Xiao-Liang;DUAN Yuan-Yuan;YOU Jing-Mao(Institute of Chinese Herbal Medicines of Hubei Academy of Agricultural Sciences,Enshi,Hubei 445000,China;Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430070,China;Hunan Agricultural University,Changsha,Hunan 410125,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第3期566-579,共14页
Mycosystema
基金
国家中药材产业技术体系(CARS-21)
湖北省民族专项(2018AKB037)
湖北省农业科学院扶持性计划(2019fcxjh09)
湖北省农业科学院中药材研究所青年科学基金(2019ZYCJJ01)
湖北省农业科学院青年基金(2020NKYJJ20)
湖南省教育厅重点项目(17A093)。
