摘要
三萜是灵芝Ganoderma lingzhi中重要的活性物质。本研究采用液体浅层静置培养方式(LSSC)提高灵芝三萜的产量。结果表明,在T25细胞培养瓶中的最佳培养条件为初始培养体积2mL,接种量7.0g(菌体湿重/L),在48h补加2mL培养液,发酵7d,三萜产量可达(32.95±0.51)mg/g,为摇瓶培养最高产量的2.09倍。进一步经不锈钢平盘放大研究发现,三萜最高产量为(65.91±0.84)mg/g,为摇瓶培养的4.19倍。形态学研究表明,浅层静置培养过程中形成的气生菌丝是提高三萜产量的关键因素。与深层液体发酵相比,液体浅层静置培养具有生长快,三萜产量高,发酵过程不需要搅拌等优点,具有良好的工业化应用前景。
Triterpenoid was recognized as the major bioactive molecule of Ganoderma lingzhi.In this work,liquid superficial-static culture(LSSC)process was used to enhance the production of triterpenoid.After optimizing,the best condition to produce triterpenoid in LSSC was initial medium volume 2 mL,inoculum size 7.0 g/L,adding medium 2 mL in 48 h and fermentative duration 7 d.Under the optimal conditions,the triterpenoid production scaled(65.91±0.84)mg/g,being 4.19-fold that in submerged culture.The results demonstrated that aerial mycelial formation was a key factor for triterpenoid biosynthesis of G.lingzhi.Compared with submerged culture,LSSC exhibited growing fast and high triterpenoid production and productivity needless to stir or shake,and had potential for industrial application.
作者
王琼
徐萌萌
刘高强
曹春蕾
石贵阳
丁重阳
WANG Qiong;XU Meng-Meng;LIU Gao-Qiang;CAO Chun-Lei;SHI Gui-Yang;DING Zhong-Yang(Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi,Jiangsu 214122,China;National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi,Jiangsu 214122,China;Hunan Provincial Key Laboratory for Forestry Biotechnology&International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology,Central South University of Forestry and Technology,Changsha,Hunan 410004,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2021年第3期656-667,共12页
Mycosystema
基金
Supported by the National Natural Science Foundation of China(31271918)
Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX181787)
National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-22)。
关键词
灵芝
液体浅层静置培养
三萜
气生菌丝
Ganoderma lingzhi
liquid superficial-static culture
triterpenoid
aerial mycelia
