摘要
目的探究lncRNA ZFPM2-AS1在膀胱癌组织中的表达和对膀胱癌T24细胞增殖、克隆形成、迁移和侵袭的影响。方法QPCR检测膀胱癌组织和细胞系中lncRNA ZFPM2-AS1的表达;将阴性对照siRNA和lncRNA ZFPM2-AS1 siRNA转染至膀胱癌T24细胞中,qPCR检测转染后细胞中lncRNA ZFPM2-AS1表达,MTT检测细胞增殖,克隆形成检测细胞克隆形成能力,细胞划痕检测细胞迁移,Transwell检测细胞侵袭,Western blot检测细胞中Wnt/β-catenin信号通路蛋白表达。结果膀胱癌组织中lncRNA ZFPM2-AS1的水平高于癌旁组织;与正常膀胱上皮细胞SV-HUC-1相比,T24、J82、56373种膀胱癌细胞株中lncRNA ZFPM2-AS1表达水平均高于正常膀胱上皮细胞,且差异有统计学意义(P<0.01)。与沉默对照组相比,沉默ZFPM2-AS1组T24细胞中lncRNA ZFPM2-AS1表达显著降低(P<0.01),细胞增殖、克隆形成、迁移和侵袭能力显著降低(P<0.01),细胞中Wnt/β-catenin信号通路蛋白β-catenin及其下游蛋白c-Myc和Cyclin D1表达显著降低,p-β-catenin蛋白表达明显增加,差异有统计学意义(P<0.01)。结论LncRNA ZFPM2-AS1在膀胱癌中高表达,沉默LncRNA ZFPM2-AS1能够抑制膀胱癌细胞增殖、克隆形成、迁移和侵袭,其机制可能与抑制Wnt/β-catenin信号通路有关。
Objective To investigate the expression of lncRNA ZFPM2-AS1 in bladder cancer tissue and its effect on the proliferation,clone formation,migration and invasion of bladder cancer T24 cells.Methods QPCR was used to detect the expression of lncRNA ZFPM2-AS1 in bladder cancer tissues and cell lines.Negative control siRNA and lncRNA ZFPM2-AS1 siRNA were transfected into bladder cancer T24 cells.Cell proliferation was detected by using MTT,cell clone formation was used to determine cell clone formation ability,cell scratches were used to detect cell migration,Transwell was used to detect cell invasion,and Western blot was used to detect Wnt/β-catenin signaling protein expression in cells.Results The level of LNCRNA ZFPM2-AS1 in bladder cancer tissue was higher than that in para-cancer tissue;compared with normal bladder epithelial cells(SV-HUC-1),the expression level of LNCRNA ZFPM2-AS1 in T24,J82 and 5637 bladder cancer cell lines was significantly higher than that in normal bladder epithelial cells(P<0.01).Compared with the silencing control group,the expression of lncRNA ZFPM2-AS1 in T24 cells of the silent ZFPM2-AS1 group was significantly reduced(P<0.01),and the capabilities of cell proliferation,colony formation,migration,and invasion were significantly reduced(P<0.01).The protein levels of Wnt/β-catenin signaling pathway proteinβ-catenin and its downstream proteins c-Myc and Cyclin D1 were significantly reduced,and the expression of p-β-catenin protein was significantly increased,the difference was statistically significant(P<0.01).Conclusion LncRNA ZFPM2-AS1 is highly expressed in bladder cancer,silencing lncRNA ZFPM2-AS1 can inhibit the cell proliferation,clone formation,migration and invasion of bladder cancer cells,which may be related to the inhibition of Wnt/β-catenin signaling pathway.
出处
《实用癌症杂志》
2021年第4期540-544,共5页
The Practical Journal of Cancer
