摘要
目的:基于网络药理学探讨"桑枝-桂枝"药对治疗类风湿关节炎的主要活性成分、主要靶点基因以及关键通路,研究作用机制。方法:使用TCMSP数据库和Swisstargetprediction数据库筛选药对活性成分和靶点,使用GeneCards和OMIM数据库获取类风湿关节炎靶点基因,获取交集基因,即"桑枝-桂枝"药对治疗类风湿关节炎的预测靶点基因。通过Cytoscape 3.7.2软件构建"药物-活性成分-靶点"网络和"药物-活性成分-靶点-疾病"网络。使用String平台构建PPI网络并通过Cytoscape 3.7.2软件分析筛选关键生物过程。在Metascape网站进行预测靶点基因的GO分析和KEGG通路分析,使用Omicshare平台绘制气泡图,同时利用Cytoscape 3.7.2软件构建"药物-成分-靶标-通路"网络。结果:共得到12个活性成分,85个预测靶点,126条KEGG通路,1 537条GO功能。"桑枝-桂枝"药对治疗类风湿关节炎的活性成分主要为山奈酚、β-谷甾醇、肉桂酸等,主要靶点基因可能为HSP90AB1、RELA、AKT1、TNF、TLR4、PTGS2等,主要KEGG通路为TNF信号通路、雌激素通路、HIF-1信号通路、NF-κB信号通路、PI3K-Akt信号通路等。主要GO功能分析为细胞对氧化应激的反应、对脂多糖的反应,对细胞因子介导通路关系。结论:本研究说明"桑枝-桂枝"药对是通过多化合物-多靶点-多通路的调控方式来治疗类风湿关节炎,并希望为后续研究中医药治疗类风湿关节炎提供一定的理论依据。
Objective:To explore the main active components,main target genes and key pathways of“mulberry twig-cassia twig”in the treatment of rheumatoid arthritis based on network pharmacology.Methods:The active components and targets of drug pairs were screened through TCMSP database and Swisstargetprediction database,and the target genes of rheumatoid arthritis were obtained through GeneCards and OMIM database,and the overlapping genes were obtained,namely,the predictive target genes of“mulberry twig-cassia twig”medicine for the treatment of rheumatoid arthritis.The“drug-active ingredient-target”network and“drug-active ingredient-target-disease”network were constructed by Cytoscape 3.7.2 software.The PPI network was constructed by String platform and the key biological processes were analyzed and screened by Cytoscape 3.7.2 software.The GO analysis and KEGG pathway analysis of predictive target genes were carried out on the Metascape website,and the bubble diagram was drawn by Omicshare platform.At the same time,those genes were used to construct the“drug-component-target-pathway”network by Cytoscape 3.7.2 software.Results:A total of 12 active components,85 predictive targets,126 KEGG pathways,and 1537 GO functions were obtained.The main active components of“mulberry twig-cassia twig”in the treatment of rheumatoid arthritis were kaempferol,beta-sitosterol and cinnamic acid,and the main target genes might be HSP90AB1,RELA,AKT1,TNF,TLR4,PTGS2,et al.The main KEGG pathways were TNF signal pathway,estrogen pathway,HIF-1 signal pathway,NF-κB signal pathway,PI3K-Akt signal pathway et al.The main GO function analysis was the response to lipopolysaccharide,response to oxidative stress,cytokine-mediated signaling pathway.Conclusion:This study shows that the“mulberry twig-cassia twig”drug pair is able to treat rheumatoid arthritisthrough the regulation of multip-compound,multi-target,multi-pathway,providing a theoretical basis for the follow-up study of traditional Chinese medicine in the treatment of rheumatoid arthritis.
作者
曹晔文
肖瑶
许涛
王欣妍
李露
曹炜
CAO Ye-wen;XIAO Yao;XU Tao;WANG Xin-yan;LI Lu;CAO Wei(Beijing University of Chinese Medicine,Beijing 100029,China;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《海南医学院学报》
CAS
2021年第8期592-602,共11页
Journal of Hainan Medical University
基金
国家重点研发计划资助项目(2018YFC1705500)。
关键词
网络药理学
桑枝
桂枝
类风湿关节炎
Network pharmacology
Mulberry twig
Cassia twig
Rheumatoid arthritis
