摘要
目的建立同时测定香薷中柠檬烯和芳樟醇含量的方法。方法采用气相色谱法,以Agilent DB-1毛细管柱(30 m×0.25 mm,0.25μm)为色谱柱;程序升温,起始温度为65℃,保持1 min,以5℃/min升至120℃,再以18℃/min升至250℃,保持5 min;进样口温度为230℃;以氮气为载气,体积流量1.0 ml/min;FID检测器温度为250℃。结果柠檬烯在42.36~847.20μg/ml(r=0.9999,n=6),芳樟醇在36.12~722.40μg/ml(r=0.9998,n=6)范围内线性关系良好,柠檬烯和芳樟醇平均回收率分别为97.10%、96.78%,RSD分别为1.71%和2.50%。结论建立的香薷中柠檬烯和芳樟醇含量测定方法,简便、准确、稳定,可用于评价香薷的质量。
Objective A GC method for simultaneous determination of Limonene and Linalool in Mosla chinensis Maxim was established.Methods The Agilent DB-1 capillary column(30 m×0.25 mm,0.25μm)was used as the chromatographic column by GC;temperature programmed,starting temperature was 65℃,maintained for 1 minute,5℃/min to 120℃,then 18℃/min to 250℃for 5 minutes;injection port temperature was 230℃;nitrogen was used as carrier gas,volume flow rate was 1.0 ml/min;FID detector temperature was 250℃.Results Limonene showed a good linear relationship in the range of 42.36~847.20μg/ml(r=0.9999,n=6),the average recovery was 97.10%and RSD was 1.71%.Linalool showed a good linear relationship in the range of 36.12~722.40μg/ml(r=0.9998,n=6),the average recovery was 96.78%and RSD was 2.50%.Conclusion The established method for the determination of Limonene and Linalool in Mosla chinensis Maxim was accurate,stable and reproducible,and could be used to evaluate the quality of Mosla chinensis Maxim.
作者
朱云
ZHU Yun(Department of Pharmacy,Lianyungang affiliated Hospital,Nanjing University of traditional Chinese Medicine,Lianyungang 222002,China)
出处
《中国处方药》
2021年第4期41-43,共3页
Journal of China Prescription Drug
关键词
GC
香薷
柠檬烯
芳樟醇
含量测定
GC
Mosla chinensis Maxim
Limonene,Linalool
Determination of content
