摘要
目的:建立百合药材高效液相色谱法(HPLC)指纹图谱及王百合苷B、王百合苷C、王百合苷E的定量方法,对不同产地百合的质量进行较全面评价。方法:采用Agilent Eclipse Plus C 18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.3%磷酸水溶液为流动相梯度洗脱,指纹图谱及含量测定检测波长为250 nm,柱温为30℃,体积流量为1.0 mL·min^(-1)。采用相似度评价(SA)、主成分分析(PCA)、随机森林分析(RFA)对21批百合药材指纹图谱进行研究。结果:建立了百合药材的HPLC指纹图谱,确定了31个共有峰;相似度和PCA结果显示,不同产地百合存在差异,进而利用随机森林算法将湖南产区和其他4个主要产地的百合样品进行区分。结论:可为全面、有效评价百合药材的质量提供参考。
Objective:To establish a method for HPLC fingerprint and quantitation of regalosides B,regalosides C,regalosides E in Lilium lancifolium,and provide a comprehensive evaluation of the drugs from different habitats.Methods:HPLC analysis was performed on an Agilent Eclipse Plus C 18 column(250 mm×4.6 mm,5μm),with a mobile phase consisting of acetonitrile-0.3%phosphoric acid at a flow rate of 1.0 mL·min^(-1).The fingerprint detection wavelength was 250 nm.The column temperature was controlled at 30℃.Similarity analysis(SA),principal component analysis(PCA)and random forest algorithm(RFA)were applied to investigate the fingerprints of 21 batches of L.lancifolium.Results:HPLC fingerprint of L.lancifolium was established and 31 common peaks were designated.The results of SA and PCA revealed the differences among the samples from various regions.The results of random forest algorithm distinguished the samples of Hunan from those of others.Conclusion:This study provides a reference for the comprehensive and effective evaluation of the quality of L.lancifolium.
作者
杨扬宇
陈林
唐雪阳
蔡萍
唐正平
张水寒
YANG Yang-yu;CHEN Lin;TANG Xue-yang;CAI Ping;TANG Zheng-ping;ZHANG Shui-han(Hunan Academy of Chinese Medicine,Changsha 410013,China;Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Chunguang Jiuhui Modern Chinese Medicine Co.,Ltd.,Liuyang 410300,China)
出处
《中国现代中药》
CAS
2021年第3期470-474,484,共6页
Modern Chinese Medicine
基金
国家中药标准化项目(ZYBZH-Y-HUN-21)
国家自然科学基金项目(81673585)
中央本级重大增减支项目(2060302-1607-01)。
