摘要
目的:采用网络药理学方法研究知柏地黄丸治疗性早熟的关键靶点和潜在分子机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)以吸收、分布、代谢和排泄(ADME)参数筛选活性化合物,并预测活性成分靶点;通过GeneCards、OMIM数据库获取性早熟相关靶点,两者相互映射得到交集靶点;采用Cytoscape 3.3.0软件构建成分-靶点网络;采用STRING数据库构建蛋白质相互作用网络;采用R软件进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析。结果:共获得知柏地黄丸中与性早熟相关的潜在活性成分63个[槲皮素、山柰素、β-谷甾醇、脱水淫羊藿素、(S)-氢化小檗碱等],潜在靶点70个[前列腺素内过氧化物合酶2(PTGS2)、PTGS1、β-2肾上腺素能受体(ADRB2)、孕酮受体(PGR)、雄激素受体(AR)等],主要涉及信号通路7条,包括色氨酸代谢、类固醇激素生物合成信号通路等。结论:知柏地黄丸通过多成分、多靶点、多途径综合调控性早熟。
Objective:To explore the key targets and latent molecular mechanism of Zhibai Dihuang Pills for the treatment of precocious puberty by network pharmacology.Methods:The active ingredients and corresponding targets were collected by using traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)according to absorption,distribution,metabolism and excretion(ADME)parameter.The disease-related genes were retrieved from GeneCards and OMIM database by using the keyword″precocious puberty″.Then the corresponding targets were mapped to the set of disease-related genes to obtain intersection targets.The Cytoscape 3.3.0 software was used to build″ingredients-targets″network.Using STRING database to construct protein interaction network.Gene ontology(GO)function enrichment and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment were analyzed by using R software.Results:A total of 63 potential active ingredients[including quercetin,kaempfero,beta-sitosterol,anhydroicaritin,(S)-canadine and so on]were screened and 70 targets[including prostaglandin G/H synthase 2(PTGS2),PTGS1,β-2 adrenergic receptor(ADRB2),progesterone receptor(PGR),androgen receptor(AR)and so on]were identified.There are 7 signaling pathways related to disease,such as tryptophan metabolism and steroid hormone biosynthesis signaling pathways.Conclusion:Zhibai Dihuang Pills systemically regular material basis and biologic mechanism of precocious puberty though multi-ingredient,multi-target and multi-channel.
作者
蔡锶
黄钢花
CAI Si;HUANG Gang-hua(Guangzhou University of Chinese Medicine,Guangzhou 510000,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510000,China)
出处
《中国现代中药》
CAS
2021年第3期475-484,共10页
Modern Chinese Medicine
关键词
知柏地黄丸
儿童
性早熟
网络药理学
Zhibai Dihuang Pills
child
precocious puberty
network pharmacology
