摘要
目的:研究谷物发酵物对产后缺乳小鼠促进泌乳的功效及作用机制。方法:选择分娩时间相差不超过24 h的KM小鼠,随机分为缺乳模型组、正常组、雌二醇组(17α-雌二醇,1 g/kg)和谷物发酵物组(24 mL/kg),每组6只。产后第3 d起,除正常组外,其余各组均采用溴隐亭(1.6 mg/kg)建立产后缺乳模型,共10 d。观察仔鼠、母鼠体重变化,每日泌乳量变化,ELISA法检测血浆催乳素(PRL)水平、HE染色观察乳腺组织学形态变化,免疫组化法检测催乳素受体(PRLR)、β-酪蛋白(β-casein)在乳腺组织中的表达。结果:正常组、雌二醇组及谷物发酵物组仔鼠窝重增加量,泌乳量,血浆PRL水平,乳腺中PRLR和β-casein的表达均高于缺乳模型组(P<0.05),母鼠体重减少量低于缺乳模型组(P<0.05),谷物发酵物组与缺乳模型组相比乳腺小叶面积明显变大,小叶间结缔组织和脂肪组织明显减少,腺泡大量增生,小叶内腺泡腔恢复到正常水平以上。结论:谷物发酵物对溴隐亭诱导的产后缺乳母鼠有促进泌乳作用,泌乳作用机制可能与增加PRL及其受体、β-casein表达有关。
Objective:To investigate the effect and mechanism of cereal fermentation on promoting lactation on mice with postpartum hypogalactia.Methods:Selected KM mice which deliver baby mice within 24 hours and divide them into groups randomly:model group,normal group,estradiol group(1 g/kg),and cereal fermentation group(24 mL/kg),6 mice in each group.Except the control group,from the 3rd day after postpartum,bromocriptine(1.6 mg/kg)was used to establish a postpartum milk loss model for a total of 10 days.Observe weight changes in filial mice and mother mice,and daily lactation volume,detect plasma prolactin(PRL)levels by enzyme-linked immunosorbent assay(ELISA),observe changes in breast histology by HE staining,and detect prolactin receptor(PRLR),β-casein by immunohistochemistry(β-casein)expression in breast tissue.Results:The litter weight increase,milk production,plasma PRL level,and expression of PRLR andβ-casein in the mammary gland were higher in the normal group,the estradiol group,and the cereal fermentation group than in the model group(P<0.05).The amount of weight loss in female mice was lower than that in the model group(P<0.05).Compared with the model group,the cereal fermentation group significantly increased the leaflet area of mammary gland,and the connective tissue and adipose tissue between the leaflet significantly reduced the large number of acinar hyperplasia and the intralobular gland.The vesicles returned to above normal levels.Conclusions:Cereal fermentation can promote the lactation of bromocriptine-induced postpartum lactating mother mice.The mechanism of lactation is related to increasing the expression of PRL,PRLR andβ-casein.
作者
杨亚洁
王明英
刘红双
热依拉·吐尔逊
张烯
廖艳
YANG Ya-jie;WANG Ming-ying;LIU Hong-shuang;REYILA·Tuerxun;ZHANG Xi;LIAO Yan(College of traditional Chinese medicine,Beijing University of traditional Chinese Medicine,Beijing,102446,China;Da Lian You Ming House Co.,Ltd.,Dalian,Liaoning,116000,China)
出处
《现代生物医学进展》
CAS
2021年第6期1014-1018,1027,共6页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81503633)
北京中医药大学校级科研纵向发展基金项目(2019-2XF2JJ-121)。
关键词
谷物发酵物
产后缺乳
催乳素
受体
Β-酪蛋白
Cereal fermentation
Postpartum hypogalactia
Prolactin
Receptor
β-casein
