摘要
目的研究鸢尾素在脓毒症大鼠头端延髓腹外侧区(RVLM)的心血管效应。方法将15只雄性SD大鼠随机分为空白对照组、假手术组和脓毒症组,每组5只,免疫组化法检测鸢尾素在RVLM表达情况。以盲肠结扎穿刺法建立大鼠脓毒症模型,将造模成功的28只雄性SD大鼠随机分为4组,每组7只,对照组RVLM预先注射人工脑脊液;实验组分别在RVLM注射10、100和1000pmol鸢尾素,信号记录分析仪记录大鼠的血压和心率。结果脓毒症大鼠RVLM鸢尾素表达明显降低(P<0.05)。单侧RVLM微量注射鸢尾素后血压升高、心率加快(P<0.05)。结论脓毒症时鸢尾素在RVLM表达降低导致心血管作用减弱,鸢尾素在中枢的水平变化与脓毒症病理过程相关。
Objective To study the cardiovascular effects of irisin within the rostral ventrolateral medulla(RVLM)in sepsis rats.Methods Fifteen male SD rats were randomly divided into control group,sham operation group and sepsis group.The expression of irisin in the RVLM was detected by immunohistochemistry.Septic rat model was established with cecal ligation and puncture(CLP)method.Twenty-eight male SD rats were randomly divided into four groups equally.The control group rats were pre-injected with artificial cerebrospinal fluid(aCSF)in RVLM,and the rats of experimental groups were micro-injected in RVLM with different consentration irisin by 10,100,1000pmol,respectively.The blood pressure(BP)and heart rate(HR)were recorded by the Signal recording analyzer.Results The expression of irisin the RVLM of rats in the sepsis group was significantly decreased compared with the normal control group(P<0.05).Unilateral microinjection of irisin into the RVLM significantly incrceased BP and HR of septic rats(P<0.05).Conclusion In the RVLM of sepsis rats,irisin produced hypertension and accelerated heart rate.Irisin is probably involved in the pathological process of sepsis by low expression in the RVLM.
作者
赫曼
杜杰
杨磊
李斌
杨毅
徐时
柴琛
He Man;Du Jie;Yang Lei(Department of Anesthesiology,People′s Hospital of SND,Jiangsu 215000,China)
出处
《医学研究杂志》
2021年第3期100-104,共5页
Journal of Medical Research
基金
甘肃省自然科学基金资助项目(17JR5RA263)
江苏省苏州高新区医疗卫生科技计划重点项目(2019Z003)
江苏省苏州高新区人民医院科学创新基金资助项目(SGY2020D01)。
关键词
鸢尾素
脓毒症
头端延髓腹外侧区
血压
心率
Irisin
Sepsis
Rostral ventrolateral medulla(RVLM)
Blood pressure(BP)
Heart rate(HR)
