摘要
目的:建立HPLC同时测定黄蜀葵花冠中芦丁、金丝桃苷和槲皮素3种活性成分的分析方法。方法:采用Waters C18色谱柱(4.6 mm×150 mm,2.7μm),乙腈-0.2%磷酸溶液作为流动相,梯度洗脱,柱温35℃,流速0.7 mL/min,进样量10μL,检测波长256 nm。结果:芦丁、金丝桃苷、槲皮素分别在0.0005~0.0100 mg/mL(r=0.99995)、0.00115~0.02300 mg/mL(r=0.99995)、0.00313~0.06300 mg/mL(r=0.99979)范围内线性关系良好,平均加样回收率分别为96.01%(RSD=0.87%)、99.60%(RSD=0.90%)、96.92%(RSD=1.72%)。结论:本法操作简便,稳定性好,可作为黄蜀葵全花的质量控制以及同时分析黄蜀葵全花中芦丁、金丝桃苷、槲皮素的有效手段。
Objective:An HPLC method for the simultaneous determination of rutin,hyperoside and quercetin from the Corolla of Abelmoschus manihot(L.)full flower was established in this paper.Methods:the Waters C18 column(4.6 mm×150 mm,2.7μm)was adopted to separate the samples with a gradient elution consisting of acetonitrile-0.2%phosphoric acid solution mobile phase at a flow of 0.7 mL/min.the column temperature was 35℃,and the detection wavelength was 256 nm,the sample volume was 10μL.Results:The three active components showed good linear relationship respectively over the concentration range of 0.0005~0.0100 mg/mL(r=0.9999)、0.00115~0.02300 mg/mL(r=0.9999)、0.00313~0.06300 mg/mL(r=0.9997),the average recovery was 96.01%(RSD=0.87%)、99.60%(RSD=0.90%)、96.92%(RSD=1.72%).Conclusion:The developed method can be used as an effective means of simultaneous rutin,hyperoside and quercetin analysis.
作者
朱俊豪
贾红倩
张彩梅
颜军
何钢
ZHU Junhao;JIA Hongqian;ZHANG Caimei;YAN Jun;HE Gang(Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department,Sichuan Industrial Institute of Antibiotics,School of Pharmacy,Chengdu University,Sichuan Chengdu 610106;Beijing Tianheng Junwei Medical Technology Development Co.,Ltd.,Beijing 102600)
出处
《生物化工》
2021年第2期1-4,共4页
Biological Chemical Engineering
基金
四川省中医药管理局科研专项资助项目(NO.2020JC0157)
关键词
HPLC
黄蜀葵花冠
芦丁
金丝桃苷
槲皮素
HPLC
Abelmoschus manihot(L.)full flower
rutin
hyperoside
quercetin
