摘要
具有几十余种化学成分的丹参粉是典型的多组分物质,应用传统二维荧光光谱难以对其作定量分析。为了研究多组分共存的丹参粉水溶液的荧光特征,利用三维同步荧光光谱法结合平行因子分析法(PARAFAC)、分半信度法(split half method)、残差平方和(sum of squared error),解析光谱重叠严重的丹参粉水溶液荧光光谱。经上述方法分析确定丹参粉水溶液的平行因子模型组分数为4,其相应组分荧光特征为:第1组分荧光特征λ_(Ex)=470~480 nm,λ_(Em)=520~530 nm;第2组分荧光特征λ_(Ex)=400~410 nm,λ_(Em)=490~500 nm;第3组分荧光特征λ_(Ex)=370~380 nm,λ_(Em)=460~470 nm;第4组分荧光特征λ_(Ex)=560~570 nm,λ_(Em)=620~630 nm。其中第2组分的荧光特征可能是丹酚酸B的荧光特征。实验结果表明,在多组分共存且有较多干扰物质时,三维同步荧光结合平行因子算法可以简化光谱分析,较好地表征丹参的整体荧光特性。本研究为多组分共存的中药材分析提供了建议与帮助。
Radix Salviae miltiorrhizae powder contains dozens of chemical constituents and is a typical multicomponent substance that is difficult to analyze quantitatively using traditional two-dimensional fluorescence spectroscopy.In this study,three-dimensional synchronous fluorescence combined with parallel factor algorithm,the split half method,and sum of squared error analysis were used to study the fluorescence characteristics of multicomponent Radix Salviae miltiorrhizae powder.The parallel factor model of a Radix Salviae miltiorrhizae powder solution was identified as four components.The corresponding component fluorescence characteristics were as follows.The first component synchronous fluorescence peak in this region wasλ_(Ex)=470-480 nm,λ_(Em)=520-530 nm,and the second component synchronous fluorescence peak in this region was λ_(Ex)=400-410 nm,λ_(Em)=490-500 nm.The third component synchronous fluorescence peak in this region was λ_(Ex)=370-380 nm,λ_(Em)=460-470 nm,and the fourth component synchronous fluorescence peak in this region was λ_(Ex)=560-570 nm,λ_(Em)=620-630 nm.The fluorescence characteristic of the second component may be the fluorescence of salvianolic acid B.Experimental results demonstrate that the three-dimensional synchronous fluorescence method combined with the parallel factor algorithm has a distinct advantage for fluorescence spectroscopic analysis,especially for a complex multicomponent substance coupled with a more interfering substance.This study can provide a reference for the analysis of multicomponent of Chinese patent medicine.
作者
李雷
吴庆州
王涛
Li Lei;Wu Qingzhou;Wang Tao(Zijin College,Nanjing University of Science&Technology,Nanjing,Jiangsu 210023,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2021年第4期439-445,共7页
Laser & Optoelectronics Progress
关键词
光谱学
荧光光谱
三维同步
平行因子算法
丹参
光谱分析
spectroscopy
fluorescence spectroscopy
three dimensional synchronous
parallel factor algorithm
radix Salviae miltiorrhizae
spectral analysis
