摘要
旨在系统评价我国健康猪源大肠杆菌对四环素类药物耐药性的情况,为养殖业合理用药决策提供依据。计算机搜索PubMed、Web of science、SinoMed、CNKI等10个数据库。应用Revman 5.3软件进行Meta分析,并对各研究间异质性进行评价。共纳入20篇符合条件的研究,共4343株分离自健康猪源的大肠杆菌。对大肠杆菌四环素类药物耐药率进行单组率Meta分析结果显示,耐药率由高到低依次是:土霉素97.0%,其95%置信区间(CI)为90.4%~99.1%;金霉素94.7%,95%CI为91.9%~97.5%;四环素93.5%,95%CI为90.8%~95.4%;强力霉素74.4%,95%CI为58.5%~85.7%;地美环素70.6%,95%CI为63.1%~77.2%;替加环素34.2%,95%CI为31.0%~37.5%;米诺环素33.2%,95%置信区间CI为3.9%~62.4%。对亚组分析结果显示:从地区上看,四环素耐药率从高到低依次是华北99.3%、华中95.1%、华北与华中94.1%、华东92.8%、华南88.6%、西南88.4%、东北87.3%;从省份来看,除西藏自治区为40.5%外,其余10省分组都超过80%;强力霉素耐药率从高到低依次是华北98.4%、华中78.1%、西南76.6%、华南68.3%、华东61.1%;从时间上看,四环素耐药率在2005至2010、2011至2015、2016至2020的3个时间段分别为94.5%、92.3%、96.3%,强力霉素耐药率在2011至2015、2016至2020的2个时间段分别为74.4%、75.7%。结果提示,中国地区健康猪源大肠杆菌对四环素类药物普遍耐药。研究设计、地区差异、使用时间长短、饲养模式等是异质性存在的主要原因。
This experiment was conducted to systematically evaluate the resistance rates to tetracyclines of Escherichia coli(E.coli)strains isolated from healthy pigs in China and to provide a clinical reference for reasonable use of veterinary medicine.We searched ten electronic databases(PubMed,Web of science,SinoMed,CNKI and so on)and performed meta-analysis using the Revman 5.3 software.A total of 668 studies were searched,with a total of 20 articles were enrolled,and 4343 E.coli strains were isolated from healthy pigs.The drug resistance rates of E.coli strains to oxytetracycline,chlortetracycline,tetracycline,doxycycline,demecycline,tigecycline,minocycline were 97.0%(95%CI=90.4%~99.1%),94.7%(95%CI=91.9%~97.5%),93.5%(95%CI=90.8%~95.3%),74.4%(95%CI=58.5%~85.7%),70.6%(95%CI=63.1%~77.2%),34.2%(95%CI=31.0%~37.5%),33.2%(95%CI=3.9%~62.4%).Subgroups analysis showed that the resistance rate of E.coli strains to tetracycline were 99.3%,95.1%,94.1%,92.8%,88.6%,88.4%,87.3% in North China,Central China,North and Central China,South China,East China,Southwest China,Northeast China,respectively.Except for the Tibet,where the drug resistance rate of E.coli strains is 40.5%,the drug resistance rate of E.coli strains were exceeds 80% in ten different provinces.The resistance rate of E.coli strains to doxycyclinewere 98.4%,78.1%,76.6%,68.3%,61.1% in North China,Central China,Southwest China,South China,East China,respectively.In the different years,the resistance rate of E.coli strains to tetracycline were 94.5%,92.3%,96.3% in 2005 to 2010,2011 to 2015,and 2016 to 2020,respectively.the resistance rate of E.coli strains to doxycycline were 74.4%,75.7% in 2011 to 2015,2016 to 2020,respectively.The resistance rate of the E.coli strains to doxycycline were 74.4%,76.6% in 2011 to 2015,and 2016 to 2020,respectively.In conclusion,there were high drug resistance rates of the E.coli strains isolated from healthy pigs to tetracyclines antibiotics in China;and research design,regional differences,duration of antibiotics use and aquaculture modes were the main reasons for the existence of heterogeneity.
作者
周玲
犹银俊
龚才伟
吕世明
谭艾娟
郑汝青
王剑
廖书丹
ZHOU Ling;YOU Yinjun;GONG Caiwei;LYU Shiming;TAN Aijuan;ZHENG Ruqing;WANG Jian;LIAO Shudan(College of Animal Science,Guizhou University,Guiyang 550025,China;Animal Disease Prevention and Control Station of Qiannan State,Qiannan State,558000,China;College of Life Science,Guizhou University,Guiyang 550025,China)
出处
《畜牧与兽医》
北大核心
2021年第4期61-68,共8页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31760749)
贵州省科技计划项目(黔科合支撑[2018]2276)
黔南州科技计划项目(黔南科合农字(2018)5号)。
