摘要
目的研究黄连-肉桂对db/db小鼠血糖、肠道菌群及脂多糖(LPS)的影响。方法取6只db/m小鼠为正常组,将30只db/db小鼠随机分成模型组、二甲双胍组、黄连组、肉桂组、黄连-肉桂组各6只。正常组和模型组给予生理盐水灌胃,其余组给予相应药物灌胃8周。灌胃前(0周)及灌胃后每周测量各组小鼠的体重,在灌胃前及灌胃4周、8周后分别测量各组小鼠的血糖;灌胃8周后采集各组小鼠粪便,16S-rDNA高通量测序对肠道菌群进行基因测序;眼球采血,酶联免疫法检测血清中LPS含量。结果各药物组小鼠体重增长趋势均减缓,黄连-肉桂组体重增长减缓最明显。各药物组小鼠血糖呈明显下降趋势,黄连-肉桂组血糖下降趋势最明显;灌胃8周后黄连组和黄连-肉桂组小鼠血糖均明显低于二甲双胍组和肉桂组(P均<0.05),黄连组和黄连-肉桂组比较差异无统计学意义(P>0.05)。与模型组及二甲双胍组比较,黄连组、肉桂组、黄连-肉桂组小鼠粪便中后壁杆菌门(Firmicutes)丰度明显下降,其中黄连-肉桂组下降最明显;黄连-肉桂组拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、变形杆菌门(Proteobacteria)及疣微菌门(Verrucomicrobia)丰度明显增加。灌胃8周后各药物组血清LPS含量均较模型组明显降低(P均<0.05),各药物组间比较差异均无统计学意义(P均>0.05),但黄连-肉桂组最低。结论黄连-肉桂可以有效降低db/db小鼠血糖,其降糖机制可能与有效调节小鼠肠道菌群及减少LPS释放入血有关。
Objective It is to study the effects of coptis-cinnamon on blood sugar,intestinal microflora and lipopolysaccharide(LPS)in db/db mice.Methods Six db/m mice were taken as normal group,and another 30 db/db mice were randomly divided into model group,metformin group,coptis group,cinnamon group,coptis-cinnamon group,6 mice in each group.The normal group and the model group were given normal saline by gavage,and the other groups were treated with corresponding drugs by gavage for 8 weeks.The body weight of the mice in each group was measured before(0 week)and every week after gavage.The blood glucose of the mice in each group was measured before gavage and 4 weeks and 8 weeks after gavage;after 8 weeks of intragastric administration,the feces of the mice in each group were collected,the gene sequence of the intestinal flora was detected by 16S-rDNA high-throughput sequencing;the blood was collected from the eyeballs to detected the LPS content in the serum by enzyme-linked immunoassay.Results The increase trend of body weight of the mice in each treatment group all slowed down,and the slowing was the most obvious in coptis-cinnamon group.The blood glucose of mice in each treatment group showed a significant downward trend,and the downward trend of blood sugar of coptis-cinnamon group was the most obvious.After 8 weeks of intragastric administration,the blood glucose of mice in the coptis group and coptis-cinnamon group was significantly lower than that of the metformin group and cinnamon groups(all P<0.05),there was no significant difference between the coptis group and coptis-cinnamon group(P>0.05).Compared with the model group and the metformin group,the abundance of Firmicutes in the feces of mice in the coptis group,cinnamon group and coptis-cinnamon group was decreased significantly,and the decrease in the coptis-cinnamon group was the most significant;the abundance of Bacteroidetes,Actinobacteria,Proteobacteria and Verrucomicrobia was increased significantly in coptis-cinnamon group.After 8 weeks of intragastric administration,the content of LPS in serum in each treatment group was significantly lower than that of the model group(all P<0.05),and there was no significant difference among the treatment groups(all P>0.05),but the that in the coptis-cinnamon group was the lowest.Conclusion Coptis-cinnamon can effectively reduce blood sugar in db/db mice,and its hypoglycemic mechanism may be related to the effective regulation of the intestinal flora of mice and the reduction of LPS release into the blood.
作者
穆国华
赵宗江
蒋里
赵进喜
MU Guohua;ZHAO Zongjiang;JIANG Li;ZHAO Jinxi(Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China;Beijing University of Chinese Medicine, Beijing 100029, China)
出处
《现代中西医结合杂志》
CAS
2021年第13期1369-1374,共6页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金资助项目(8177151329)。
关键词
黄连
肉桂
2型糖尿病
肠道菌群
内毒素
脂多糖
coptis
cinnamon
type 2 diabetes
intestinal flora
endotoxin
lipopolysaccharide
