摘要
目的:研究胃癌组织中角蛋白23(KRT23)的表达情况及其临床意义。方法:收集4例新鲜的胃癌组织及其对应的癌旁组织,85例胃癌蜡块和与其匹配的癌旁组织,通过Western blot检测KRT23蛋白表达水平。并应用免疫组化方法检测85例胃癌组织和与其配对的癌旁组织蜡块中KRT23蛋白的表达情况,分析KRT23蛋白表达与胃癌患者临床病理特征之间的关系。采用Cox比例风险模型及Kaplan-Meier生存分析预后相关性。结果:与癌旁组织相比,胃癌组织中KRT23蛋白表达水平明显升高(P<0.001)。KRT23蛋白表达水平与TNM分期(P=0.009)、淋巴结转移(P<0.001)有关。Kaplan-Meier生存分析提示:与KRT23阴性表达相比,KRT23阳性表达患者的总生存时间显著缩短(P<0.05)。Cox回归分析提示:KRT23阳性表达(P=0.047)、淋巴结转移(P=0.01)、TNM分期高(P=0.024)是影响胃癌患者预后的独立危险因素。结论:KRT23在胃癌患者组织中高表达,可能在胃癌发病和进展过程中发挥重要作用,为胃癌诊断、治疗和预后评估提供了新的思路和靶点。
Objective:To study the expression of keratin 23 typeⅠ(KRT23)in gastric cancer tissue and its clinical significance.Methods:Four cases of fresh gastric cancer tissue and their corresponding adjacent tissue,85 cases of paraffin tissue block and their corresponding adjacent tissue were collected,and the expression level of KRT23 protein was detected by Western blot.In addition,immunohistochemical method was used to detect the expression of KRT23 protein in 85 cases of gastric cancer tissue and paracancerous tissue paraffin block,and analyze the relationship between KRT23 protein expression and clinicopathological features of gastric cancer patients.The Cox proportional hazards model and Kaplan-Meier survival were used to analyze the prognostic correlation.Results:Compared with adjacent tissues,the expression level of KRT23 in gastric cancer tissues was significantly higher(P<0.001).The expression level of KRT23 protein was related to TNM staging(P=0.009)and lymphatic metastasis(P<0.001).Kaplan-Meier survival analysis showed that compared with KRT23 negative expression,the overall survival time of patients with KRT23 positive expression was significantly shorter(P<0.05).Cox regression analysis indicated that KRT23 positive(P=0.047),lymph node metastasis(P=0.01),and high TNM stage(P=0.024)were independent risk factors affecting the prognosis of gastric cancer patients.Conclusion:KRT23 is highly expressed in the tissue of gastric cancer patients,and may play an important role in the pathogenesis and progression of gastric cancer.It provides new ideas and targets for the diagnosis,treatment and prognosis evaluation of gastric cancer.
作者
杭群
左芦根
骆杰
朱冰
刘牧林
HANG Qun;ZUO Lugen;LUO Jie;ZHU Bing;LIU Mulin(Gastrointestinal Department,the First Affiliated Hospital of Bengbu Medical College,Anhui Bengbu 233000,China)
出处
《现代肿瘤医学》
CAS
北大核心
2021年第9期1531-1534,共4页
Journal of Modern Oncology
基金
国家自然科学基金资助项目(编号:81700476)
蚌埠医学院科技发展基金项目(编号:BYKF1743)。
关键词
KRT23
胃癌
临床病理学特征
预后
KRT23
gastric cancer
clinicopathological features
prognosis
