摘要
目的:考察和评价灭菌方式对人参粉杀菌效果的影响。方法:建立HPLC指纹图谱,测定皂苷含量,通过PLS-DA分析、聚类分析、相似度评价,结合HPLC法对不同方式灭菌样品进行评价,并对样品进行微生物数量检测及6个月的稳定性考察。结果:指纹图谱指认10个共有峰,未灭菌、湿热灭菌样品的相似度为0.981~0.995,辐照灭菌和紫外灭菌样品相似度为0.971~0.977,臭氧灭菌样品相似度为0.784~0.863,通过PLS-DA分析指认3个色谱峰可有效区分不同灭菌方式的样品。随着辐照灭菌剂量的升高,人参皂苷Rg1、Re、Rb1、Rf、Ro的含量均明显升高。不同灭菌方式人参粉放置6个月与0月相比各皂苷含量差异无明显变化。结论:通过指纹图谱结合指标成分含量分析,可更好的评价人参粉灭菌效果,为确定人参粉的灭菌工艺提供依据。
Objective:To investigate and evaluate the sterilization methods on the sterilization effect of Ginseng powder.Methods:HPLC fingerprint was established to determine the contents of saponins.The samples were evaluated by PLS-DA analysis,cluster analysis,similarity evaluation,combined with HPLC method,and the number of microorganisms in the samples,the stability for 6 months were analyzed.Rresults:The fingerprint identified 10 common peaks,the similarity of unsterilized and humid heart sterilized samples was 0.981~0.995.The similarity of irradiation sterilization and ultraviolet sterilization samples were 0.971~0.977.The similarity of ozone sterilized samples was 0.784~0.863.Three chromatographic peaks were identified by PLS-DA analysis to distinguish the samples with different sterilization methods.With the increasing of irradiation sterilization dose,the contents of ginsenoside Rg1,Re,Rb1,Rf and Ro significantly increased.There was no significant difference in the contents of ginsenoside between different sterilization methods after 6 months and 0 months.Conclusion:The quality of ginseng powder could be better evaluated by fingerprint analysis combined with index composition analysis,which would provide the basis for determining the sterilization process of ginseng powder.
作者
白羽辛
王玥玥
贡济宇
何文媛
李妍
蔡广知
BAI Yuxin;WANG Yueyue;GONG Jiyu;HE Wenyuan;LI Yan;CAI Guangzhi(Changchun University of Chinese Medicine,Changchun 130000,China)
出处
《食品工业科技》
CAS
北大核心
2021年第10期229-235,共7页
Science and Technology of Food Industry
基金
吉林省科技发展计划项目(20200404035YY)
全国中药特色技术传承人才培训项目(T20194828003)。
关键词
人参粉
灭菌效果
指纹图谱
人参皂苷
微生物数量
稳定性
ginseng powder
sterilization
HPLC fingerprint
ginsenoside
microbial population
stability
