摘要
基于转录组测序数据,该研究采用PCR扩增方法从蒙古冰草中克隆AmWRKY1-like的全长cDNA,并对其进行了生物信息学和亚细胞定位分析,为深入研究AmWRKY1-like在蒙古冰草干旱胁迫响应中的调控作用提供理论基础。结果发现:成功克隆得到了AmWRKY1-like基因,其开放阅读边框(ORF)为1182 bp、编码393个氨基酸,含有典型的WRKY转录因子保守结构域;qRT-PCR表明,在自然干旱和15%PEG-6000模拟干旱下,蒙古冰草叶片中AmWRKY1-like均可诱导表达,较对照呈下调表达趋势;亚细胞定位表明,AmWRKY1-like基因在细胞核中特异表达。
Basic on transcriptome data,we cloned the full length cDNAs of transcription factors genes AmWRKY1-like from A.mongolicum by PCR amplification,and their bioinformatic analysis and localization analysis were performed.These will contribute to study the biological functions of AmWRKY1-like responding to drought stress in A.mongolicum.The results showed that:AmWRKY1-like gene were successful cloned,and the open reading frame(ORF)was 1182 bp and encoded 393 amino acids,and contains a typical conserved domain of WRKY transcription factors.AmWRKY1-like gene was down-regulated in leaves of A.mongolicum compared with CK under natural drought and 15%PEG-6000 drought conditions via qRT-PCR.Subcellular localization assay showed that AmWRKY1-like was specifically expressed in the nucleus.
作者
陈燕福
范菠菠
赵彦
武小娟
赵娜勤
马艳红
CHEN Yanfu;FAN Bobo;ZHAO Yan;WU Xiaojuan;ZHAO Naqin;MA Yanhong(Agricultural College of Inner Mongolia Agricultural University, Hohhot 010019, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2021年第3期386-391,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31860670)
内蒙古自然科学基金(2018MS03016)。
